Lee Sung Ho, Jin Wei-Peng, Seo Na Ri, Pang Kang-Mi, Kim Bongju, Kim Soung-Min, Lee Jong-Ho
Dental Research Institute, Department of Oral and Maxillofacial Surgery, Seoul National University School of Dentistry, Seoul, Korea.
Dental Life Science Research Institute, Clinical Translational Research Center for Dental Science, Seoul National University Dental Hospital, Seoul, Korea.
Neural Regen Res. 2017 Apr;12(4):629-636. doi: 10.4103/1673-5374.205104.
Several studies have shown that fibroblast growth factor-2 (FGF2) can directly affect axon regeneration after peripheral nerve damage. In this study, we performed sensory tests and histological analyses to study the effect of recombinant human FGF-2 (rhFGF2) treatment on damaged mental nerves. The mental nerves of 6-week-old male Sprague-Dawley rats were crush-injured for 1 minute and then treated with 10 or 50 μg/mL rhFGF2 or PBS in crush injury area with a mini Osmotic pump. Sensory test using von Frey filaments at 1 week revealed the presence of sensory degeneration based on decreased gap score and increased difference score. However, at 2 weeks, the gap score and difference score were significantly rebounded in the mental nerve crush group treated with 10 μg/mL rhFGF2. Interestingly, treatment with 10 μg/mL rhFGF had a more obviously positive effect on the gap score than treatment with 50 μg/mL rhFGF2. In addition, retrograde neuronal tracing with Dil revealed a significant increase in nerve regeneration in the trigeminal ganglion at 2 and 4 weeks in the rhFGF2 groups (10 μg/mL and 50 μg/mL) than in the PBS group. The 10 μg/mL rhFGF2 group also showed an obviously robust regeneration in axon density in the mental nerve at 4 weeks. Our results demonstrate that 10 μg/mL rhFGF induces mental nerve regeneration and sensory recovery after mental nerve crush injury.
多项研究表明，成纤维细胞生长因子2（FGF2）可直接影响周围神经损伤后的轴突再生。在本研究中，我们进行了感觉测试和组织学分析，以研究重组人FGF-2（rhFGF2）治疗对受损颏神经的影响。将6周龄雄性Sprague-Dawley大鼠的颏神经挤压损伤1分钟，然后用微型渗透泵在挤压损伤区域给予10或50μg/mL rhFGF2或PBS治疗。在第1周使用von Frey细丝进行感觉测试，基于间隙评分降低和差异评分增加揭示了感觉退化的存在。然而，在第2周，用10μg/mL rhFGF2治疗的颏神经挤压组的间隙评分和差异评分显著反弹。有趣的是，与50μg/mL rhFGF2治疗相比，10μg/mL rhFGF治疗对间隙评分有更明显的积极影响。此外，用Dil进行逆行神经元示踪显示，在第2周和第4周，rhFGF2组（10μg/mL和50μg/mL）三叉神经节中的神经再生比PBS组显著增加。10μg/mL rhFGF2组在第4周时颏神经的轴突密度也显示出明显更强的再生。我们的结果表明，10μg/mL rhFGF可诱导颏神经挤压损伤后的颏神经再生和感觉恢复。
