Scarano Antonio, Crincoli Vito, Di Benedetto Adriana, Cozzolino Valerio, Lorusso Felice, Podaliri Vulpiani Michele, Grano Maria, Kalemaj Zamira, Mori Giorgio, Grassi Felice Roberto
Department of Medical, Oral and Biotechnological Sciences and CeSI-Met, University of Chieti-Pescara, Chieti, Italy.
Interdisciplinary Department of Medicine, University of Bari "A. Moro", Bari, Italy.
Stem Cells Int. 2017;2017:9082869. doi: 10.1155/2017/9082869. Epub 2017 May 2.
. Adding stem cells to biodegradable scaffolds to enhance bone regeneration is a valuable option. Different kinds of stem cells with osteoblastic activity were tested, such as bone marrow stromal stem cells (BMSSCs). . To assess a correct protocol for osteogenic stem cell differentiation, so BMSSCs were seeded on a bone porcine block (BPB). . Bone marrow from six minipigs was extracted from tibiae and humeri and treated to isolate BMSSCs. After seeding on BPB, critical-size defects were created on each mandible of the minipigs and implanted with BPB and BPB/BMSSCs. After three months, histomorphometric analysis was performed. . Histomorphometric analysis provided percentages of the three groups. Tissues present in control defects were 23 ± 2% lamellar bone, 28 ± 1% woven bone, and 56 ± 4% marrow spaces; in BPB defects were 20 ± 5% BPB, 32 ± 2% lamellar bone, 24 ± 1% woven bone, and 28 ± 2% marrow spaces; in BPB/BMSSCs defects were 17 ± 4% BPB/BMSSCs, 42 ± 2% lamellar bone, 12 ± 1% woven bone, and 22 ± 3% marrow spaces. . BPB used as a scaffold to induce bone regeneration may benefit from the addition of BDPSCs in the tissue-engineered constructs.
将干细胞添加到可生物降解支架中以增强骨再生是一种有价值的选择。人们测试了不同种类具有成骨活性的干细胞，如骨髓间充质干细胞（BMSSCs）。为评估成骨干细胞分化的正确方案，将BMSSCs接种在猪骨块（BPB）上。从六只小型猪的胫骨和肱骨中提取骨髓并进行处理以分离BMSSCs。接种到BPB上后，在小型猪的每个下颌骨上制造临界尺寸缺损，并植入BPB和BPB/BMSSCs。三个月后，进行组织形态计量学分析。组织形态计量学分析给出了三组的百分比。对照缺损处的组织为23±2%的板层骨、28±1%的编织骨和56±4%的骨髓腔；BPB缺损处为20±5%的BPB、32±2%的板层骨、24±1%的编织骨和28±2%的骨髓腔；BPB/BMSSCs缺损处为17±4%的BPB/BMSSCs、42±2%的板层骨、12±1%的编织骨和22±3%的骨髓腔。用作诱导骨再生支架的BPB可能会受益于在组织工程构建物中添加BDPSCs。
