Barker R A, Maxwell P H, Hong C P, Cordery M C, Chrystie I L
Department of Virology, St Thomas's Hospital, London, UK.
Trans R Soc Trop Med Hyg. 1988;82(6):898-901. doi: 10.1016/0035-9203(88)90034-x.
Over a period of 2 months, 35 of 69 (51%) cases of juvenile diarrhoea studied in eastern Malaysia were associated with rotavirus excretion; rotavirus associated diarrhoea occurred most commonly in the 6-24 month age group. Polyacrylamide gel electrophoresis (PAGE) of genome ribonucleic acid showed that only 4 rotavirus electropherotypes could be detected. Of those, 2 predominated and 2 were detected only once each; one of these may have been a reassortment of the two predominant electropherotypes. Analysis of the clinical features of patients excreting rotavirus subgroup 1 or 2, determined by PAGE, demonstrated that rotavirus subgroup 1 was associated with more hypotonic dehydration and need for intravenous therapy: lethargy was significantly more common among those excreting rotavirus subgroup 2.
在两个月的时间里，马来西亚东部研究的69例青少年腹泻病例中有35例（51%）与轮状病毒排泄有关；轮状病毒相关性腹泻最常发生在6至24个月龄组。基因组核糖核酸的聚丙烯酰胺凝胶电泳（PAGE）显示，仅能检测到4种轮状病毒电泳型。其中，2种为主型，另外2种各仅检测到一次；其中一种可能是两种主型的重配体。通过PAGE确定排泄1型或2型轮状病毒亚组患者的临床特征分析表明，1型轮状病毒与更多的低渗性脱水及静脉治疗需求有关：排泄2型轮状病毒亚组的患者中嗜睡明显更常见。
