Yang Jianhua, Ju Bowei, Yan Yao, Xu Huanhuan, Wu Shanshan, Zhu Dandan, Cao Dandan, Hu Junping
Department of Pharmacy, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
Department of Natural Medicines, College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
Exp Ther Med. 2017 May;13(5):2423-2428. doi: 10.3892/etm.2017.4254. Epub 2017 Mar 22.
The present study aimed to investigate the neuroprotective effects of phenylethanol glycosides (PhGs) on HO- and β-amyloid peptide (Aβ)-induced injury of PC12 cells as an model of Alzheimer's disease (AD). The optimal induction conditions were established through screening of various incubation times and concentrations. PC12 cells were treated with 0.5 µM Aβ and HO in the presence of PhGs for 24 h and the cell viability was then evaluated by an MTT assay; lactate dehydrogenase (LDH) release and malondialdehyde (MDA) content were also measured. The optimal conditions for establishing the AD model were the treatment of PC12 cells with 0.5 µM Aβ for 48 h, or with 25 µM HO dissolved in DMEM with PBS. PhGs at concentrations of 5, 25 and 50 µg/ml increased the viability and decreased LDH and MDA release by PC12 cells injured with Aβ or HO. In conclusion, the model of Aβ- and HO-induced PC12 cell injury was successfully established. PhGs were shown to have a significant neuroprotective effect against Aβ- or HO-induced cell injury.
本研究旨在探讨苯乙醇苷（PhGs）对以HO和β-淀粉样肽（Aβ）诱导的PC12细胞损伤的神经保护作用，该损伤作为阿尔茨海默病（AD）的模型。通过筛选不同的孵育时间和浓度建立最佳诱导条件。在存在PhGs的情况下，用0.5 μM Aβ和HO处理PC12细胞24小时，然后通过MTT法评估细胞活力；还测量了乳酸脱氢酶（LDH）释放和丙二醛（MDA）含量。建立AD模型的最佳条件是用0.5 μM Aβ处理PC12细胞48小时，或用溶解于含PBS的DMEM中的25 μM HO处理。浓度为5、25和50 μg/ml的PhGs提高了被Aβ或HO损伤的PC12细胞的活力，并降低了LDH和MDA释放。总之，成功建立了Aβ和HO诱导的PC12细胞损伤模型。结果表明，PhGs对Aβ或HO诱导的细胞损伤具有显著的神经保护作用。
