Dănilă Oana-Ofelia, Berghian Alexandra Sevastre, Dionisie Vlad, Gheban Dan, Olteanu Diana, Tabaran Flaviu, Baldea Ioana, Katona Gabriel, Moldovan Bianca, Clichici Simona, David Luminita, Filip Gabriela Adriana
Department of Physiology, "Iuliu Hatieganu" University of Medicine & Pharmacy, 1-3 Clinicilor Street, Cluj-Napoca, 400006, Romania.
Department of Morphopathology, "Iuliu Hatieganu" University of Medicine & Pharmacy, 35 Clinicilor Street, Cluj-Napoca, 400006, Romania.
Nanomedicine (Lond). 2017 Jun;12(12):1455-1473. doi: 10.2217/nnm-2017-0029. Epub 2017 Jun 1.
To investigate the comparative effects of prenatal exposure to silver nanoparticles (AgNPs) functionalized with citrate and polyphenols on spatial cognition and also on nitro-oxidative stress and apoptosis in the hippocampus and cerebellum of offsprings.
MATERIALS & METHODS: Pregnant Wistar rats were orally administered substances from day 10 of gestation until day 21. Six weeks postpartum, six male offsprings from each group were used for behavioral evaluation and determination of oxidative stress and apoptosis.
Both groups exhibited hyperactivity and anxiety especially after AgNPs-Sambucus nigra L. administration. AgNPs-S. nigra L. group showed increase in induced nitric oxide synthase activity and decrease in superoxide dismutase activity and apoptosis in the hippocampus, while AgNPs-citrate coated administration exerted a moderate toxicity and induced apoptosis.
AgNPs functionalized with natural extracts had a lower toxicity than citrate-coated silver particles.
研究产前暴露于柠檬酸盐和多酚功能化的银纳米颗粒（AgNPs）对后代空间认知以及对海马体和小脑的硝基氧化应激和细胞凋亡的比较影响。
怀孕的Wistar大鼠从妊娠第10天至第21天口服给予物质。产后六周，每组六只雄性后代用于行为评估以及氧化应激和细胞凋亡的测定。
两组均表现出多动和焦虑，尤其是在给予黑接骨木AgNPs后。黑接骨木AgNPs组海马体中诱导型一氧化氮合酶活性增加，超氧化物歧化酶活性降低，细胞凋亡减少，而柠檬酸盐包被的AgNPs给药产生中度毒性并诱导细胞凋亡。
用天然提取物功能化的AgNPs比柠檬酸盐包被的银颗粒毒性更低。
