Zhang Jie, Xie Youneng, Zuo Jun, Li Jiaxin, Wei Qiuping, Yu Zhiming, Tang Zhangui
School of Materials Science and Engineering, Central South University, Changsha, 410083, PR China; Xiangya Stomatological Hospital, Central South University, Changsha 410078, PR China; Xiangya Hospital, Central South University, Changsha, 410078, PR China.
School of Materials Science and Engineering, Central South University, Changsha, 410083, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:1187-1194. doi: 10.1016/j.msec.2017.04.119. Epub 2017 Apr 21.
Sandblast and acid-etching (SLA) is the most prevalent treatment to titanium implants, while residual sand particles are inevitably introduced on SLA titanium surfaces. NHOH and HO mixture was used to etch titanium plates (E) and titanium bars (EB), aiming at substituting sandblast procedure. To study the effects of different scale rough structures on cell response of Human osteoblast-like cells (MG63), traditional HSO and HCl mixture was also used to further etch the titanium plates above (DE). Holes of 10-20μm were obtained on E and DE surfaces, which are very close to the size of osteoblasts. Surfaces with micro/nano and micro/submicro hierarchical structures were obtained on the treated titanium. As-prepared E, DE and EB surfaces are hydrophilic, while only EB stayed hydrophilic after 5days' exposure to air. MG63 cultured on E and EB surfaces showed higher proliferation rate and attachment area than on DE and P surfaces. E and DE showed higher alkaline phosphatases (ALP) activity after 7 and 14days of osteoinduction, while EB showed the highest osteopontin (OPN) and bone sialoprotein (BSP) production after 21days of osteoinduction. These results indicate that E and EB surfaces boost the proliferation and osteogenic differentiation of MG63 without introducing sand particles. This is a promising treatment to titanium implant.
喷砂和酸蚀（SLA）是钛植入物最普遍的处理方法，而在SLA钛表面不可避免地会引入残留的砂粒。使用NHOH和HO混合物对钛板（E）和钛棒（EB）进行蚀刻，旨在替代喷砂工艺。为了研究不同尺度粗糙结构对人成骨样细胞（MG63）细胞反应的影响，还使用传统的HSO和HCl混合物对上述钛板进行进一步蚀刻（DE）。在E和DE表面获得了10 - 20μm的孔，这与成骨细胞的大小非常接近。在处理后的钛表面获得了具有微/纳米和微/亚微分级结构的表面。制备的E、DE和EB表面具有亲水性，而在暴露于空气5天后只有EB保持亲水性。在E和EB表面培养的MG63显示出比在DE和P表面更高的增殖率和附着面积。在成骨诱导7天和14天后，E和DE显示出更高的碱性磷酸酶（ALP）活性，而在成骨诱导21天后，EB显示出最高的骨桥蛋白（OPN）和骨唾液蛋白（BSP）产量。这些结果表明，E和EB表面在不引入砂粒的情况下促进了MG63的增殖和成骨分化。这是一种有前景的钛植入物处理方法。
