Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan; Indonesian Sweetener and Fiber Crops Research Institute, Jalan Raya Karangploso Km 9, Malang, East Java 65152, Indonesia.
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
Bioresour Technol. 2017 Dec;245(Pt B):1393-1399. doi: 10.1016/j.biortech.2017.05.146. Epub 2017 May 24.
A transformant of Moorella thermoacetica was constructed for thermophilic ethanol production from lignocellulosic biomass by deleting two phosphotransacetylase genes, pdul1 and pdul2, and introducing the native aldehyde dehydrogenase gene (aldh) controlled by the promoter from glyceraldehyde-3-phosphate dehydrogenase. The transformant showed tolerance to 540mM and fermented sugars including fructose, glucose, galactose and xylose to mainly ethanol. In a mixed-sugar medium of glucose and xylose, all of the sugars were consumed to produce ethanol at the yield of 1.9mol/mol-sugar. The transformant successfully fermented sugars in hydrolysate prepared through the acid hydrolysis of lignocellulose to ethanol, suggesting that this transformant can be used to ferment the sugars in lignocellulosic biomass for ethanol production.
一株嗜热醋酸菌（Moorella thermoacetica）转化体被构建出来，用于从木质纤维素生物质中生产高温乙醇。该转化体通过删除两个磷酸转乙酰酶基因 pdul1 和 pdul2，并引入受甘油醛-3-磷酸脱氢酶启动子控制的天然醛脱氢酶基因（aldh）来实现。该转化体对 540mM 的耐受能力，并发酵包括果糖、葡萄糖、半乳糖和木糖在内的糖，主要产物为乙醇。在葡萄糖和木糖的混合糖培养基中，所有的糖都被消耗掉，以 1.9mol/mol-糖的产率生成乙醇。该转化体成功地发酵了木质纤维素酸水解制备的水解液中的糖，产生乙醇，这表明该转化体可用于发酵木质纤维素生物质中的糖来生产乙醇。
