M Brandão Clenilma, R Dos Santos Djanira, Silva Lucas G P, Ferreira Mirla C, de F Mesquita Joyce M, P Souza Melissa, Holanda Carlos A, S Gonçalves Renato, V Costa Emmanoel, de C Marques Georgiana E, de M Teles Rogério, B Cavalcante Kiany S
Postgraduate Program in Chemistry, Associative Doctorate UFMA-IFMA, Federal Institute of Maranhão, São Luís-Monte Castelo Campus, São Luís, MA 65030-005, Brazil.
Laboratory of Microbiological Analysis, Department of Chemistry, Federal Institute of Maranhão, São Luís-Monte Castelo Campus, São Luís, MA 65030-005, Brazil.
J Agric Food Chem. 2025 Aug 6;73(31):19327-19339. doi: 10.1021/acs.jafc.5c04690. Epub 2025 Jul 24.
Essential oil (EO) nanoformulations are emerging as green nanotechnology strategies against and s mosquitoes, which are vectors of arboviruses such as dengue. For the first time, we investigated the larvicidal influence of polysorbate (PS) against mosquito using simplex centroid design (SCD) in the formulation of EO nanoemulsions derived from the species (Plantaginaceae), a plant native to the Cerrado region of Maranhão, Brazil. In addition, we present the ecotoxicological profile of the formulation against microcrustaceans. EO was extracted by hydrodistillation, and its chemical profile was analyzed by GC-MS-FID and NMR (H, C, DEPT-135, and 2D). The nanoformulations were characterized by DLS, PDI, ZP, and TEM. The chemical profile indicated the presence of compounds such as fenchol, fenchyl acetate, caryophyllene, and caryophyllene oxide. The SCD contributed to the development of nanostructured formulations with spheroidal morphology, demonstrating effective larvicidal action against mosquitoes with lower toxicity than commercial chemical larvicides.
精油（EO）纳米制剂正作为对抗蚊子的绿色纳米技术策略而兴起，蚊子是登革热等虫媒病毒的传播媒介。我们首次使用单纯形重心设计（SCD）研究了聚山梨酯（PS）对蚊子的杀幼虫影响，该设计用于制备源自巴西马拉尼昂塞拉多地区本土植物（车前科）的EO纳米乳液。此外，我们还展示了该制剂对微型甲壳类动物的生态毒理学概况。通过水蒸馏法提取EO，并通过气相色谱 - 质谱 - 火焰离子化检测（GC - MS - FID）和核磁共振（H、C、DEPT - 135和二维）分析其化学概况。通过动态光散射（DLS）、多分散指数（PDI）、zeta电位（ZP）和透射电子显微镜（TEM）对纳米制剂进行表征。化学概况表明存在诸如葑醇、葑基乙酸酯、石竹烯和石竹烯氧化物等化合物。SCD有助于开发具有球形形态的纳米结构制剂，对蚊子显示出有效的杀幼虫作用，且毒性低于商业化学杀幼虫剂。
