Bio/Polymer Research Group, Centre for Advanced Macromolecular Design, School of Biotechnology & Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Regen Med. 2012 Mar;7(2):159-66. doi: 10.2217/rme.12.6.
To generate a comprehensive profile of the protein composition of xenogeneic biomaterial, derived from porcine urinary bladder matrix (UBM).
MATERIALS & METHODS: Tunica layers and muscularis mucosa were removed from bladders using mechanical delamination. UBM was prepared using a solution of peracetic acid in ethanol, lyophilized then milled into powder. UBM biomaterial was subjected to tryptic digests and components separated using high-performance liquid chromatography with an ion trap mass spectrometer and identified through databases.
A repertoire of 129 proteins with neurotrophic, antiangiogenic and tumor-suppressive activities and those associated with tissue remodeling and wound repair were identified.
This study provides the first insight into the complex nature of the UBM and how its application may be tailored for specific applications in regenerative medicine. We propose that the UBM be further investigated for reconstructive and regenerative remodeling of cardiac and dermal tissues, as well as peripheral nerves.
生成源自猪膀胱基质（UBM）的异种生物材料的蛋白质组成的综合图谱。
使用机械分层法从膀胱中去除膜层和黏膜肌层。UBM 使用过乙酸在乙醇中的溶液制备，冻干后研磨成粉末。UBM 生物材料进行胰蛋白酶消化，使用高效液相色谱与离子阱质谱联用分离成分，并通过数据库进行鉴定。
鉴定出了 129 种具有神经营养、抗血管生成和肿瘤抑制作用的蛋白质，以及与组织重塑和伤口修复相关的蛋白质。
本研究首次深入了解了 UBM 的复杂性质，以及如何针对再生医学中的特定应用来调整其应用。我们建议进一步研究 UBM 在心脏和皮肤组织以及周围神经的重建和再生重塑中的应用。
