Liang Shuang, Niu Ying Jie, Shin Kyung-Tae, Cui Xiang-Shun
Department of Animal Science,Chungbuk National University,Cheongju,Chungbuk,361-763,Republic of Korea.
Microsc Microanal. 2017 Aug;23(4):849-858. doi: 10.1017/S1431927617000617. Epub 2017 Jun 7.
Coenzyme Q10 (Q10) plays an important role in the cellular antioxidant system by protecting the cells from free-radical oxidative damage and apoptosis. In the present study, we have investigated the effect of Q10 on the preimplantation development of porcine parthenogenetic embryos, as well as the underlying mechanism. The results showed that 100 μM was the optimal concentration of Q10, which resulted in significantly increased cleavage and blastocyst formation rates and improvement of blastocyst quality. Q10 improved the blastocyst hatching rate and cellular proliferation rate in hatching blastocysts and increased the expression of hatching-related genes. Furthermore, Q10 not only decreased reactive oxygen species production, DNA damage levels, and apoptosis in the blastocysts from H2O2-induced oxidative injury, but also maintained mitochondrial function. Taken together, these results indicate that Q10 has beneficial effects on the development of porcine parthenogenetic embryos by preventing oxidative damage and apoptosis.
辅酶Q10（Q10）通过保护细胞免受自由基氧化损伤和凋亡，在细胞抗氧化系统中发挥重要作用。在本研究中，我们研究了Q10对猪孤雌胚胎植入前发育的影响及其潜在机制。结果表明，100μM是Q10的最佳浓度，其导致显著提高的卵裂率和囊胚形成率以及囊胚质量的改善。Q10提高了囊胚孵化率和孵化囊胚中的细胞增殖率，并增加了孵化相关基因的表达。此外，Q10不仅降低了H2O2诱导的氧化损伤的囊胚中的活性氧产生、DNA损伤水平和凋亡，还维持了线粒体功能。综上所述，这些结果表明Q10通过预防氧化损伤和凋亡对猪孤雌胚胎的发育具有有益作用。
