Sanchis-Mora Sandra, Chang Yu-Mei, Abeyesinghe Siobhan, Fisher Amy, Volk Holger A, Pelligand Ludovic
Comparative Biomedical Sciences, Royal Veterinary College, University of London, London, UK.
Research Office, Royal Veterinary College, University of London, London, UK.
Vet Anaesth Analg. 2017 May;44(3):600-614. doi: 10.1016/j.vaa.2016.09.004. Epub 2017 Feb 24.
To study the feasibility and test-retest repeatability of a sensory threshold examination protocol (STEP) and report the quantitative sensory threshold distributions in healthy dogs.
Prospective, observational, cohort study.
Twenty-five healthy client-owned dogs.
Tactile sensitivity test (TST) (von Frey filaments), mechanical thresholds (MT with 2, 4 and 8 mm probes), heat thresholds (HT) and responsiveness to cold stimulus (CT at 0 °C) were quantitatively assessed for five body areas (BAs; tibias, humeri, neck, thoracolumbar region and abdomen) in a randomized order on three different occasions. Linear mixed model and generalized linear mixed models were used to evaluate the effects of body weight category, age, sex, BA, occasion, feasibility score and investigator experience. Test-retest repeatability was evaluated with the intra-class correlation coefficient.
The STEP lasted 90 minutes without side effects. The BA affected most tests (p ≤ 0.001). Higher thresholds and longer cold latencies were scored in the neck (p ≤ 0.024) compared to other BAs. Weight category affected all thresholds (p ≤ 0.037). Small dogs had lower MT (∼1.4 N mean difference) and HT (1.1 °C mean difference) than other dogs (p ≤ 0.029). Young dogs had higher HT than adults (2.2 °C mean difference) (p = 0.035). Gender also affected TST, MT and HT (p < 0.05) (females versus males: TST odds ratio = 0.5, MT = 1.3 N mean difference, HT = 2.2 °C mean difference). Repeatability was substantial to moderate for all tests, but poor for TST. There was no difference in thresholds between occasions, except for CT. Test-retest repeatability was slightly better with the 2 mm MT probe compared to other diameters and improved with operator experience.
The STEP was feasible, was well tolerated and showed substantial test-retest repeatability in healthy dogs. Further validation is needed in dogs suffering pain.
研究一种感觉阈值检查方案（STEP）的可行性和重测重复性，并报告健康犬的定量感觉阈值分布情况。
前瞻性、观察性队列研究。
25只健康的客户拥有的犬。
对五个身体部位（胫骨、肱骨、颈部、胸腰椎区域和腹部）进行触觉敏感性测试（TST）（用von Frey细丝）、机械阈值（用2、4和8毫米探头的MT）、热阈值（HT）和对冷刺激的反应性（0°C时的CT）评估，在三个不同时间以随机顺序进行。使用线性混合模型和广义线性混合模型来评估体重类别、年龄、性别、身体部位、时间、可行性评分和研究者经验的影响。用组内相关系数评估重测重复性。
STEP持续90分钟，无副作用。身体部位对大多数测试有影响（p≤0.001）。与其他身体部位相比，颈部的阈值更高，冷潜伏期更长（p≤0.024）。体重类别影响所有阈值（p≤0.037）。小型犬的MT（平均差异约1.4 N）和HT（平均差异1.1°C）低于其他犬（p≤0.029）。幼犬的HT高于成年犬（平均差异2.2°C）（p = 0.035）。性别也影响TST、MT和HT（p < 0.05）（雌性与雄性相比：TST优势比 = 0.5，MT = 平均差异1.3 N，HT = 平均差异2.2°C）。所有测试的重复性从中等到良好，但TST较差。除CT外，不同时间的阈值无差异。与其他直径相比，2毫米MT探头的重测重复性稍好，且随着操作者经验的增加而提高。
STEP在健康犬中是可行的，耐受性良好，重测重复性良好。在疼痛犬中还需要进一步验证。
