Knazovicky David, Helgeson Erika S, Case Beth, Thomson Andrea, Gruen Margaret E, Maixner William, Lascelles B Duncan X
Comparative Pain Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Department of Biostatistics, University of North Carolina, Chapel Hill, NC, USA.
Vet Anaesth Analg. 2017 May;44(3):615-624. doi: 10.1016/j.vaa.2016.08.008. Epub 2017 Jan 10.
To evaluate replicate effects and test-retest reliability of mechanical and thermal quantitative sensory testing (QST) in normal dogs and dogs with osteoarthritis (OA)-associated pain.
A prospective clinical study.
A total of 54 client owned dogs (OA, n=31; controls, n=23).
Mechanical [electronic von Frey (EVF) and blunt pressure] and thermal (hot and cold) sensory thresholds were obtained in dogs with OA-associated pain and control dogs at two visits, 7 days apart, to assess test-retest reliability. Thresholds were measured at the OA-affected joint (hip or stifle), over the tibial muscle and over the midpoint of the metatarsals. Five replicates were obtained for each modality at each site bilaterally.
Overall, there was no significant effect of replicates on QST response. EVF thresholds were significantly lower at the second visit in OA dogs at the affected and metatarsal sites (p=0.0017 and p=0.0014, respectively). Similarly for control dogs, EVF thresholds were significantly lower at the second visit at the metatarsal site (p=0.001). Significantly higher hot thermal latencies were seen in OA dogs at the affected and tibial testing sites (p=0.014 and p=0.012, respectively), and in control dogs at the tibial site (p=0.004).
In QST, a replicate does not show a strong effect. However, QST results show variability over time, particularly for EVF and hot thermal stimuli.
If QST is to be used clinically to evaluate a sensitized state, the variability over time needs to be accounted for in the study design.
评估正常犬和患骨关节炎（OA）相关疼痛犬的机械性和热定量感觉测试（QST）的重复性效应和重测信度。
一项前瞻性临床研究。
总共54只客户拥有的犬（OA组，n = 31；对照组，n = 23）。
对患OA相关疼痛的犬和对照犬进行两次检查，间隔7天，以获取机械性（电子von Frey [EVF]和钝性压力）和热（热和冷）感觉阈值，评估重测信度。在OA受累关节（髋部或 stifle关节）、胫骨肌肉上方和跖骨中点测量阈值。在每个部位双侧对每种模式进行5次重复测量。
总体而言，重复测量对QST反应无显著影响。OA犬在受累部位和跖骨部位第二次检查时EVF阈值显著降低（分别为p = 0.0017和p = 0.0014）。同样，对照犬在跖骨部位第二次检查时EVF阈值也显著降低（p = 0.001）。OA犬在受累部位和胫骨测试部位的热潜伏期显著延长（分别为p = 0.014和p = 0.012），对照犬在胫骨部位也有显著延长（p = 0.004）。
在QST中，重复测量未显示出强烈影响。然而，QST结果随时间存在变异性，特别是对于EVF和热刺激。
如果要在临床中使用QST评估敏感状态，在研究设计中需要考虑随时间的变异性。
