粘质沙雷氏菌脯氨酸高产菌株：通过增加渗透胁迫增强脯氨酸类似物介导的生长抑制作用
Proline-hyperproducing strains of Serratia marcescens: enhancement of proline analog-mediated growth inhibition by increasing osmotic stress.
作者信息
Sugiura M, Kisumi M
出版信息
Appl Environ Microbiol. 1985 Apr;49(4):782-6. doi: 10.1128/aem.49.4.782-786.1985.
Abstract
Proline-producing strains of Serratia marcescens were more osmotolerant than wild-type strains. Growth inhibition by proline analogs was significantly enhanced by increasing the osmotic stress of the medium. Mutants resistant to azetidine-2-carboxylate were derived from a proline-producing strain, SP126, under a high osmotic condition. One of the mutants, strain SP187, produced 56 mg of L-proline per ml of medium containing sucrose and urea. This amount was ca. 3 times larger than that produced by strain SP126. The intracellular glutamate content which decreased in strain SP126 was restored in strain SP187. The glutamate dehydrogenase level of strain SP187 was 5 times higher than that of strain SP126.
摘要
产脯氨酸的粘质沙雷氏菌菌株比野生型菌株具有更强的耐渗透压能力。脯氨酸类似物对生长的抑制作用会随着培养基渗透压的升高而显著增强。在高渗条件下，从产脯氨酸菌株SP126中获得了对氮杂环丁烷 -2-羧酸具有抗性的突变体。其中一个突变体SP187菌株，在含有蔗糖和尿素的培养基中每毫升可产生56毫克L-脯氨酸。这个产量约是SP126菌株产量的3倍。在SP126菌株中降低的细胞内谷氨酸含量在SP187菌株中得以恢复。SP187菌株的谷氨酸脱氢酶水平比SP126菌株高5倍。
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本文引用的文献
1
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Glutamine and proline accumulation by Staphylococcus aureus with reduction in water activity.金黄色葡萄球菌在水分活度降低时谷氨酰胺和脯氨酸的积累
Appl Environ Microbiol. 1982 Jun;43(6):1501-3. doi: 10.1128/aem.43.6.1501-1503.1982.
4
A third L-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength.鼠伤寒沙门氏菌中的第三种L-脯氨酸通透酶，其在高渗透压强度的培养基中发挥作用。
J Bacteriol. 1982 Sep;151(3):1433-43. doi: 10.1128/jb.151.3.1433-1443.1982.
5
Proline over-production results in enhanced osmotolerance in Salmonella typhimurium.脯氨酸过量产生可增强鼠伤寒沙门氏菌的渗透压耐受性。
Mol Gen Genet. 1981;182(1):82-6. doi: 10.1007/BF00422771.
6
Salmonella typhimurium mutants with altered glutamate dehydrogenase and glutamate synthase activities.具有改变的谷氨酸脱氢酶和谷氨酸合酶活性的鼠伤寒沙门氏菌突变体。
J Bacteriol. 1980 Jan;141(1):190-8. doi: 10.1128/jb.141.1.190-198.1980.
7
Accumulation of carbohydrate by Escherichia coli B/r/1 during growth at low water activity.低水分活度下生长时大肠杆菌B/r/1中碳水化合物的积累
J Appl Bacteriol. 1982 Jun;52(3):425-34. doi: 10.1111/j.1365-2672.1982.tb05073.x.
8
Stabilization of a histidine-producing strain of Serratia marcescens.粘质沙雷氏菌组氨酸生产菌株的稳定性
Appl Environ Microbiol. 1984 Jul;48(1):43-7. doi: 10.1128/aem.48.1.43-47.1984.
9
Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
10
Sub-cloning of the wild-type proAB region of the Escherichia coli genome.大肠杆菌基因组野生型proAB区域的亚克隆
J Gen Microbiol. 1983 Oct;129(10):3215-25. doi: 10.1099/00221287-129-10-3215.
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