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锌改性硅酸钙涂层通过 TGF-β/Smad 通路促进骨质疏松兔的成骨分化和骨整合。

Zinc-modified Calcium Silicate Coatings Promote Osteogenic Differentiation through TGF-β/Smad Pathway and Osseointegration in Osteopenic Rabbits.

机构信息

Department of Orthopaedics, Changzheng Hospital of Second Military Medical University, Shanghai, 200003, China.

National Engineering Research Center for Nanotechnology, Shanghai, 200241, China.

出版信息

Sci Rep. 2017 Jun 13;7(1):3440. doi: 10.1038/s41598-017-03661-5.

DOI:10.1038/s41598-017-03661-5
PMID:28611362
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5469779/
Abstract

Surface-modified metal implants incorporating different ions have been employed in the biomedical field as bioactive dental implants with good osseointegration properties. However, the molecular mechanism through which surface coatings exert the biological activity is not fully understood, and the effects have been difficult to achieve, especially in the osteopenic bone. In this study, We examined the effect of zinc-modified calcium silicate coatings with two different Zn contents to induce osteogenic differentiation of rat bone marrow-derived pericytes (BM-PCs) and osteogenetic efficiency in ovariectomised rabbits. Ti-6Al-4V with zinc-modified calcium silicate coatings not only enhanced proliferation but also promoted osteogenic differentiation and mineralized matrix deposition of rat BM-PCs as the zinc content and culture time increased in vitro. The associated molecular mechanisms were investigated by Q-PCR and Western blotting, revealing that TGF-β/Smad signaling pathway plays a direct and significant role in regulating BM-PCs osteoblastic differentiation on Zn-modified coatings. Furthermore, in vivo results that revealed Zn-modified calcium silicate coatings significantly promoted new bone formation around the implant surface in osteopenic rabbits as the Zn content and exposure time increased. Therefore, Zn-modified calcium silicate coatings can improve implant osseointegration in the condition of osteopenia, which may be beneficial for patients suffering from osteoporosis-related fractures.

摘要

表面改性的金属植入物中加入不同的离子已经被应用于生物医学领域作为具有良好骨整合性能的生物活性牙科植入物。然而，表面涂层发挥生物活性的分子机制尚不完全清楚，而且效果也难以实现，尤其是在骨质疏松的骨骼中。在这项研究中，我们研究了两种不同锌含量的锌改性硅酸钙涂层对诱导大鼠骨髓来源周细胞（BM-PCs）成骨分化和去卵巢兔成骨效率的影响。Ti-6Al-4V 表面的锌改性硅酸钙涂层不仅增强了细胞的增殖，还促进了大鼠 BM-PCs 的成骨分化和矿化基质的沉积，随着锌含量和培养时间的增加。通过 Q-PCR 和 Western blot 研究了相关的分子机制，结果表明 TGF-β/Smad 信号通路在调节 Zn 改性涂层上 BM-PCs 成骨分化中起着直接而显著的作用。此外，体内结果表明，随着 Zn 含量和暴露时间的增加，Zn 改性硅酸钙涂层显著促进了骨质疏松兔植入物表面周围的新骨形成。因此，Zn 改性硅酸钙涂层可以改善骨质疏松条件下植入物的骨整合，这可能对患有骨质疏松性骨折的患者有益。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc0/5469779/a78394e2d9f2/41598_2017_3661_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc0/5469779/b88bc870c85b/41598_2017_3661_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc0/5469779/a78394e2d9f2/41598_2017_3661_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc0/5469779/b88bc870c85b/41598_2017_3661_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc0/5469779/53b00cbe57b7/41598_2017_3661_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc0/5469779/b329cd959348/41598_2017_3661_Fig3_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc0/5469779/a8671fa04499/41598_2017_3661_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dc0/5469779/a78394e2d9f2/41598_2017_3661_Fig7_HTML.jpg

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