Bao-Min Shi, Kun Ji, Yu-Feng Wang, Shuai Xiao, Department of General Surgery, Tongji Hospital, Medical School of Tongji University, Shanghai 200065, China.
World J Gastroenterol. 2017 May 28;23(20):3713-3720. doi: 10.3748/wjg.v23.i20.3713.
To analyze the incidence of hepatocellular carcinoma (HCC) in a population that underwent health checkups and had high serum miR-106b levels.
A total of 335 subjects who underwent checkups in the Digestive and Liver Disease Department of our hospital were randomly selected. RT-PCR was used to detect the level of miR-106b in serum samples. Laboratory and imaging examinations were carried out to confirm the HCC diagnosis in patients who had a > 2-fold change in miR-106b levels. Ultrasound-guided biopsy was also used for HCC diagnosis when necessary. On this basis, the clinical data of these subjects, including history of hepatitis virus infection, obesity, long-term history of alcohol use and stage of HCC, were collected. Then, the impact of these factors on the level of miR-106b in serum was analyzed. Furthermore, receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic efficacy of miR-106b for HCC.
A total of 35 subjects had abnormal serum miR-106b levels, of which 20 subjects were diagnosed with HCC. -test revealed that the difference in serum miR-106b level in terms of sex, age, history of hepatitis virus infection, obesity and long-term history of alcohol use was not statistically significant. However, serum miR-106b levels in patients with advanced HCC (stage III/IV) was higher than in patients with early HCC (stage I/II), and the difference was statistically significant ( = 0.000). Moreover, the ROC curve revealed that the area under the curve value for miR-106b was 0.885, which shows that serum miR-106b level has a certain clinical value for HCC diagnosis.
The random sampling survey shows that serum miR-106b level is a valuable diagnostic marker for HCC. However, the diagnostic threshold value needs to be further researched.
分析在接受健康检查且血清 miR-106b 水平较高的人群中肝细胞癌（HCC）的发病率。
随机选取我院消化肝病科 335 例体检者，采用 RT-PCR 检测血清样本中 miR-106b 水平，对 miR-106b 水平升高 2 倍以上的患者进行实验室和影像学检查以明确 HCC 诊断，必要时采用超声引导下肝活检进行 HCC 诊断。在此基础上收集这些受试者的临床资料，包括肝炎病毒感染史、肥胖、长期饮酒史及 HCC 分期，分析这些因素对血清 miR-106b 水平的影响，绘制受试者工作特征（ROC）曲线评价 miR-106b 对 HCC 的诊断效能。
共 35 例受试者血清 miR-106b 水平异常，其中 20 例诊断为 HCC。卡方检验发现，血清 miR-106b 水平在性别、年龄、肝炎病毒感染史、肥胖、长期饮酒史方面的差异无统计学意义。但晚期 HCC（Ⅲ/Ⅳ期）患者血清 miR-106b 水平高于早期 HCC（Ⅰ/Ⅱ期），差异有统计学意义（ = 0.000）。此外，ROC 曲线显示 miR-106b 的曲线下面积值为 0.885，提示血清 miR-106b 水平对 HCC 诊断具有一定的临床价值。
随机抽样调查显示血清 miR-106b 水平是 HCC 的有价值诊断标志物，但诊断界值有待进一步研究。
