Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Herbal Medicines Research Center, Student Research Committee, School of Pharmacy, Shahid Sadoughi University of Medical Science Health Services, Yazd, Iran.
BMC Complement Med Ther. 2022 Jul 30;22(1):203. doi: 10.1186/s12906-022-03676-0.
Henna (Lawsonia inermis) with anti-bacterial properties has been widely used in traditional medicine especially Persian medicine. Henna oil is suggested for diseases of infectious origin, such as cervical ulcers. Group B Streptococcus agalactiae, Pseudomonas aeruginosa and, Trichomonas vaginalis are involved in the infection of women especially cervicitis. Henna grows in dry and tropical regions. The main important landraces of henna landraces are cultivated in Kerman, Sistan and Baluchestan, Hormozgan, and Bushehr provinces in Iran. Proper use of antimicrobial agents, use of new antimicrobial strategies, and alternative methods, such as herbal methods may help reduce drug resistance in the future. This study's objective was to investigate the anti-Trichomonas vaginalis activity of three different henna landraces and antimicrobial effects against group B Streptococcus agalactiae and, Pseudomonas aeruginosa.
Total phenol content was measured by Folin ciocaltu method. Antibacterial effect of landraces of Henna against P. aeruginosa and S. agalactiae were assayed by well diffusion method and minimal inhibitory concentration assessments were done using the broth micro-dilution technique. Anti-Trichomonas effect of Henna landraces were assayed by Hemocytometery method.
Total phenol content of Shahdad, Rudbar-e-Jonub, and Qaleh Ganj was 206.51, 201.96, and 254.85 μg/ml, respectively. Shahdad, Rudbar-e-Jonub, and Qaleh Ganj had MIC against GBS at 15, 15 and, 4 μg/ml. The growth inhibition diameter of the most effective henna (Shahdad landrace) at a concentration of 20 μg/ml on P. aeruginosa was 2.46 ± 0.15 cm and in the MIC method at a concentration of 5 μg/ml of Shahdad landrace, P. aeruginosa did not grow. IC50 of shahdad Henna after 24 h, 48 h, and 72 h was 7.54, 4.83 and 20.54 μg/ml, respectively. IC50 of Rudbar-e-Jonub extract was 5.76, 3.79 and 5.77 μg/ml in different days. IC50 of Qaleh Ganj extract was 6.09, 4.08 and 5.74 μg/ml in different days.
The amount of total phenol in Qaleh Ganj was higher than the other varieties. In the well diffusion method, Qaleh Ganj was more effective against group B Streptococcus (Gram-positive bacterium) than the other two landraces, and Shahdad landrace was more effective against P. aeruginosa (Gram-negative bacterium) than other. In the MIC method, the same result was obtained as in the well diffusion method, but at a lower concentration.
具有抗菌特性的指甲花（指甲花）已在传统医学中广泛使用，尤其是在波斯医学中。指甲花油被建议用于治疗感染性疾病，如宫颈溃疡。B 群链球菌、绿脓假单胞菌和阴道毛滴虫参与了女性的感染，特别是宫颈炎。指甲花生长在干燥和热带地区。指甲花的主要重要地方品种在伊朗的克尔曼、锡斯坦和俾路支斯坦、霍尔木兹甘和布什尔省种植。适当使用抗菌药物、使用新的抗菌策略和替代方法，如草药方法，可能有助于减少未来的耐药性。本研究的目的是研究三种不同指甲花地方品种对阴道毛滴虫的抗滴虫活性以及对 B 群链球菌和绿脓假单胞菌的抗菌作用。
采用福林-希奥考尔特法测定总酚含量。采用琼脂扩散法测定指甲花地方品种对绿脓假单胞菌和 B 群链球菌的抑菌作用，采用微量肉汤稀释法测定最小抑菌浓度。采用血细胞计数法测定指甲花地方品种对阴道毛滴虫的抗滴虫作用。
Shahdad、Rudbar-e-Jonub 和 Qaleh Ganj 的总酚含量分别为 206.51、201.96 和 254.85μg/ml。Shahdad、Rudbar-e-Jonub 和 Qaleh Ganj 对 GBS 的 MIC 值分别为 15、15 和 4μg/ml。浓度为 20μg/ml 的最有效指甲花（Shahdad 地方品种）对绿脓假单胞菌的生长抑制直径为 2.46±0.15cm，在 MIC 法中，浓度为 5μg/ml 的 Shahdad 地方品种绿脓假单胞菌未生长。Shahdad 指甲花在 24、48 和 72 小时的 IC50 分别为 7.54、4.83 和 20.54μg/ml。Rudbar-e-Jonub 提取物在不同天数的 IC50 分别为 5.76、3.79 和 5.77μg/ml。Qaleh Ganj 提取物在不同天数的 IC50 分别为 6.09、4.08 和 5.74μg/ml。
Qaleh Ganj 的总酚含量高于其他品种。在琼脂扩散法中，Qaleh Ganj 对 B 群链球菌（革兰氏阳性菌）的作用比其他两种地方品种更有效，而 Shahdad 地方品种对绿脓假单胞菌（革兰氏阴性菌）的作用比其他品种更有效。在 MIC 法中，与琼脂扩散法一样，在较低浓度下也获得了相同的结果。
