Hu Xiaoxia, Wang Yingqian, Liu Haoyang, Wang Jie, Tan Yaning, Wang Fubing, Yuan Quan, Tan Weihong
Key Laboratory of Analytical Chemistry for Biology and Medicine , Ministry of Education , College of Chemistry and Molecular Sciences , Wuhan University , Wuhan , P. R. China . Email:
Department of Laboratory Medicine & Center for Gene Diagnosis , Zhongnan Hospital , Wuhan University , Wuhan , P. R. China.
Chem Sci. 2017 Jan 1;8(1):466-472. doi: 10.1039/c6sc03401b. Epub 2016 Aug 19.
Development of a portable device for the detection of multiple mRNAs is a significant need in the early diagnosis of cancer. We have designed a biochip-based mRNA detection device by combining a hydrophilic-hydrophobic micropattern with upconversion luminescence (UCL) probes. The device achieves highly sensitive detection, using the naked eye, of multiple mRNAs among patient samples. The high sensitivity is attributed to enrichment of the target concentration and a fluorescence enhancement effect. In addition, since the photonic crystal (PC) dot biochip is functionalized with dual-wavelength excitation UCL probes, two kinds of mRNAs in the heterogeneous biological samples are detected simultaneously, and the corresponding luminescence signals are captured using an unmodified camera phone. The biochip-based mRNA detection device reported here demonstrates that multiple mRNAs extracted from patient samples can be simultaneously and sensitively detected in a visual way without sophisticated instrumentation. Therefore, this device is promising for real-time detection of multiple biomarkers in patient samples, and it is anticipated that it will provide a powerful tool for convenient early diagnosis of cancer.
开发一种用于检测多种mRNA的便携式设备是癌症早期诊断的迫切需求。我们通过将亲水-疏水微图案与上转换发光（UCL）探针相结合，设计了一种基于生物芯片的mRNA检测设备。该设备可通过肉眼对患者样本中的多种mRNA进行高灵敏度检测。高灵敏度归因于目标浓度的富集和荧光增强效应。此外，由于光子晶体（PC）点生物芯片用双波长激发UCL探针进行了功能化，可同时检测异质生物样本中的两种mRNA，并使用未改装的拍照手机捕获相应的发光信号。本文报道的基于生物芯片的mRNA检测设备表明，从患者样本中提取的多种mRNA无需复杂仪器即可通过可视化方式同时进行灵敏检测。因此，该设备有望用于实时检测患者样本中的多种生物标志物，并有望为便捷的癌症早期诊断提供强大工具。
