Smith D A, Lantos P L
Acta Neuropathol. 1985;66(2):155-9. doi: 10.1007/BF00688691.
Two astrocytic immunocytochemical markers, glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS), were demonstrated in cerebellar astrocytomas. The staining pattern for both antigens was similar, but GFAP demonstrated astrocytic processes better, while GS staining was stronger in poorly fibrillated cells. The various astrocytic forms, bipolar, stellate, bi- and multi-nucleate cells, displayed different immunostaining. Rosenthal fibres also showed a varying pattern of reaction: larger fibres were entirely negative or had a narrow peripheral ring of dense reaction product, but occasional smaller fibres were positive. The varying proportion of filamentous and amorphous material within Rosenthal fibres could be responsible for this finding. The pale granular bodies possessed a GFAP-positive limiting membrane and GS-positive contents, suggestive of their astrocytic origin. Immunohistochemistry of GFAP and GS has contributed to the knowledge of cellular differentiation and secondary changes in cerebellar astrocytomas.
在小脑星形细胞瘤中证实了两种星形胶质细胞免疫细胞化学标志物，即胶质纤维酸性蛋白（GFAP）和谷氨酰胺合成酶（GS）。两种抗原的染色模式相似，但GFAP能更好地显示星形胶质细胞的突起，而GS染色在纤维形成不良的细胞中更强。各种星形胶质细胞形态，如双极、星状、双核和多核细胞，显示出不同的免疫染色。罗森塔尔纤维也表现出不同的反应模式：较大的纤维完全阴性或有狭窄的外周致密反应产物环，但偶尔较小的纤维呈阳性。罗森塔尔纤维内丝状和无定形物质比例的变化可能是这一发现的原因。苍白颗粒体有GFAP阳性的限制膜和GS阳性的内容物，提示其星形胶质细胞起源。GFAP和GS的免疫组织化学有助于了解小脑星形细胞瘤的细胞分化和继发性变化。
