Mou Qianqian, He Jingxia, Yin Rongli, Yang Bin, Fu Meihong, Fang Jing, Li Hua
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Molecules. 2017 Jun 15;22(6):1000. doi: 10.3390/molecules22061000.
Single-factor experiment and Box-Behnken design were applied to optimize the infrared-assisted extraction (IRAE) of rutin, quercetin, kaempferol, and isorhamnetin from Flos sophorae. Four factors (extract solvent, solid-liquid ration, extraction time, infrared power) affecting the extraction yield of flavonoids were studied. Under optimized conditions the extraction yield was 33.199 ± 0.24 mg/g, which substantially improved, compared with heating reflux extraction (HRE) and ultrasonic-assisted extraction (UAE), while extraction time was only 9 min. The eluents were rich in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azobis (2-methyl-propionamidine) dihydrochloride radical scavenging potential (IC of DPPH: 53.44 ± 0.01 μg/mL, oxygen radical absorbance capacity (ORAC): 3785.83 ± 52 μmol/g) than the extracts obtained by HRE and UAE. In addition, an ultra-high performance liquid chromatography method was optimized for the identification and quantification of the tested flavonoids, and the method was validated based on its correlation coefficient (r), reproducibility (RSD, n = 5), and recovery values, which were 0.9994-0.9998, 0.74-1.83%, and 97.78-102.94%, respectively. These results confirmed that high extraction yield of flavonoids results in stronger antioxidant values and response surface methodology optimization of IRAE is a promising alternative to traditional extraction techniques for flavonoids from medicinal plants.
采用单因素实验和Box-Behnken设计对槐花中芦丁、槲皮素、山奈酚和异鼠李素的红外辅助提取（IRAE）工艺进行优化。研究了影响黄酮类化合物提取率的四个因素（提取溶剂、固液比、提取时间、红外功率）。在优化条件下，提取率为33.199±0.24mg/g，与加热回流提取（HRE）和超声辅助提取（UAE）相比有显著提高，而提取时间仅为9分钟。洗脱液富含2,2-二苯基-1-苦基肼（DPPH）和2,2'-偶氮二（2-甲基丙脒）二盐酸盐自由基清除能力（DPPH的IC：53.44±0.01μg/mL，氧自由基吸收能力（ORAC）：3785.83±52μmol/g），优于HRE和UAE获得的提取物。此外，优化了一种超高效液相色谱法用于检测黄酮类化合物的鉴定和定量，并基于其相关系数（r）、重现性（RSD，n = 5）和回收率值对该方法进行了验证，相关系数分别为0.9994 - 0.9998、0.74 - 1.83%和97.78 - 102.94%。这些结果证实，黄酮类化合物的高提取率导致更强的抗氧化值，并且IRAE的响应面法优化是从药用植物中提取黄酮类化合物的传统提取技术的一种有前途的替代方法。
