a Department of Biology, Faculty of Sciences , University of Mohaghegh Ardabili , Ardabil , Iran.
b Department of Chemistry, Faculty of Sciences , University of Mohaghegh Ardabili , Ardabil , Iran.
Artif Cells Nanomed Biotechnol. 2018 Jun;46(4):730-739. doi: 10.1080/21691401.2017.1337025. Epub 2017 Jun 15.
Herein, ZnO nanoparticles were synthesized using microwave-assisted method in the presence of Vaccinium arctostaphylos L, fruits extract. The structure, size, morphology and optical properties of the samples were characterized by XRD, SEM, TEM, EDX, FT-IR, UV-vis DRS and TGA analysis. A decrease in crystallite size was observed for the biologically synthesized ZnO compared to the chemically synthesized sample. Furthermore, the existence of organic moieties over the biologically synthesized ZnO NPs was approved using characterizing methods. Then, the alloxan-induced diabetic rats were divided into not treated (diabetic control group), and the groups received: insulin, chemically synthesized ZnO, plant extract, biologically synthesized ZnO with a normal healthy control group. After treatment, fasting blood glucose (FBS), high-density lipoprotein (HDL), total triglyceride (TG), total cholesterol (TC) and insulin were measured. Analysis showed a significant decrease in FBS and increase in HDL levels in all groups under treatment. However, the results for cholesterol reduction were only significant for the group treated by biologically synthesized ZnO. Despite the changes in the triglyceride and insulin levels, the results were not significant. For all the studied parameters, bio-mediated ZnO NPs were found the most effective in treating the alloxan-diabetic rats compared to the other studied treatment agents.
在此，使用微波辅助法在越桔（Vaccinium arctostaphylos L.）果实提取物的存在下合成了 ZnO 纳米粒子。通过 XRD、SEM、TEM、EDX、FT-IR、UV-vis DRS 和 TGA 分析对样品的结构、尺寸、形态和光学性质进行了表征。与化学合成的样品相比，生物合成的 ZnO 的晶粒度减小。此外，使用表征方法证实了生物合成的 ZnO NPs 上存在有机基团。然后，将链脲佐菌素诱导的糖尿病大鼠分为未治疗（糖尿病对照组）和接受以下治疗的组：胰岛素、化学合成的 ZnO、植物提取物、生物合成的 ZnO，以及正常健康对照组。治疗后，测量空腹血糖（FBS）、高密度脂蛋白（HDL）、总甘油三酯（TG）、总胆固醇（TC）和胰岛素。分析表明，所有治疗组的 FBS 均显著降低，HDL 水平均显著升高。然而，只有生物合成的 ZnO 治疗组的胆固醇降低结果具有统计学意义。尽管甘油三酯和胰岛素水平发生了变化，但结果并不显著。对于所有研究的参数，与其他研究的治疗剂相比，生物介导的 ZnO NPs 被发现是治疗链脲佐菌素糖尿病大鼠最有效的。
