Congestrì Francesco, Pedna Maria Federica, Fantini Michela, Samuelli Michela, Schiavone Pasqua, Torri Arianna, Bertini Stefania, Sambri Vittorio
Unit of Microbiology, Greater Romagna Hub Laboratory, Pievesestina di Cesena, Italy.
Unit of Microbiology, Greater Romagna Hub Laboratory, Pievesestina di Cesena, Italy; DIMES, University of Bologna, Bologna, Italy.
Int J Infect Dis. 2017 Sep;62:1-5. doi: 10.1016/j.ijid.2017.06.012. Epub 2017 Jun 15.
The early detection of bacteraemia and fungemia is of paramount importance to guide antimicrobial therapy in septic patients. In this study the 'time to detection' (TTD) value for the new blood culture system BacT/ALERT VIRTUO (VIRTUO) was evaluated in 1462 positive clinical bottles and compared with the TTD for 1601 positive clinical bottles incubated in the BacT/ALERT 3D system (BTA-3D).
The most representative microorganisms isolated from bottles incubated in both blood culture systems were divided into eight categories (in order of frequency): coagulase-negative staphylococci (CoNS), Escherichia coli, Enterobacteriaceae (other than E. coli), Staphylococcus aureus, Enterococcus spp, viridans group streptococci, Pseudomonas aeruginosa, and Candida spp.
The comparison of TTD values for the two blood culture systems strongly indicated that growth of the first five groups listed above was detected earlier with VIRTUO than with BTA-3D (p < 0.05).
The new VIRTUO blood culture system can reduce the TTD for more than 75% of isolated microorganisms.
菌血症和真菌血症的早期检测对于指导脓毒症患者的抗菌治疗至关重要。在本研究中，对新型血培养系统BacT/ALERT VIRTUO（VIRTUO）的“检测时间”（TTD）值在1462份阳性临床培养瓶中进行了评估，并与在BacT/ALERT 3D系统（BTA - 3D）中孵育的1601份阳性临床培养瓶的TTD进行了比较。
从两种血培养系统中孵育的培养瓶中分离出的最具代表性的微生物分为八类（按频率排序）：凝固酶阴性葡萄球菌（CoNS）、大肠杆菌、肠杆菌科（除大肠杆菌外）、金黄色葡萄球菌、肠球菌属、草绿色链球菌、铜绿假单胞菌和念珠菌属。
两种血培养系统TTD值的比较强烈表明，VIRTUO比BTA - 3D更早检测到上述前五组微生物的生长（p < 0.05）。
新型VIRTUO血培养系统可降低75%以上分离微生物的TTD。
