通过计算机辅助精子分析系统（CASA）评估精子活力进行聚类分析，比较日本黑牛首次射精和第二次射精精液中的精子亚群结构。
Comparison of sperm subpopulation structures in first and second ejaculated semen from Japanese black bulls by a cluster analysis of sperm motility evaluated by a CASA system.
作者信息
Kanno Chihiro, Sakamoto Kentaro Q, Yanagawa Yojiro, Takahashi Yoshiyuki, Katagiri Seiji, Nagano Masashi
机构信息
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan.
Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan.
出版信息
J Vet Med Sci. 2017 Aug 4;79(8):1359-1365. doi: 10.1292/jvms.17-0012. Epub 2017 Jun 18.
In the present study, bull sperm in the first and second ejaculates were divided into subpopulations based on their motility characteristics using a cluster analysis of data from computer-assisted sperm motility analysis (CASA). Semen samples were collected from 4 Japanese black bulls. Data from 9,228 motile sperm were classified into 4 clusters; 1) very rapid and progressively motile sperm, 2) rapid and circularly motile sperm with widely moving heads, 3) moderately motile sperm with heads moving frequently in a short length, and 4) poorly motile sperm. The percentage of cluster 1 varied between bulls. The first ejaculates had a higher proportion of cluster 2 and lower proportion of cluster 3 than the second ejaculates.
在本研究中，利用计算机辅助精子活力分析（CASA）的数据进行聚类分析，将4头日本黑公牛第一次和第二次射精的精子根据其活力特征分为亚群。从4头日本黑公牛采集精液样本。9228条活动精子的数据被分为4类：1）非常快速且进行性运动的精子；2）头部广泛移动的快速且圆周运动的精子；3）头部在短距离内频繁移动的中度运动精子；4）活力差的精子。第1类的百分比在公牛之间有所不同。与第二次射精相比，第一次射精中第2类的比例较高，第3类的比例较低。
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