Barai Milan, Ahsan Nazmul, Paul Nilanjana, Hossain Khaled, Abdur Rashid Mohammad, Kato Masashi, Ohgami Nobutaka, Azim Akhand Anwarul
Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.
Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205, Bangladesh.
Nagoya J Med Sci. 2017 Feb;79(2):167-177. doi: 10.18999/nagjms.79.2.167.
Arsenic created a serious public health problem in Bangladesh due to its presence in groundwater and dissemination of the toxic effects to millions of people. The scarcity of the treatment options to manage this affected population has made the situation much worse. To find a promising treatment option, this study was undertaken to examine the ameliorating roles of leaf extract (SLE) against arsenic-induced toxic effects in mice. Swiss albino mice were divided into four groups where 'control' group received pure water + normal feed, 'arsenic (As)' group received sodium arsenite (NaAsO)-containing water (10 μg/g body weight/day) + normal feed, 'As+SLE' group received NaAsO-containing water + feed supplemented with SLE (50 µg/g body weight/day) and finally the 'SLE' group received pure water + feed supplemented with SLE. A gradual increase in body weight gain was observed in control mice; however, the body weight gain in As-exposed mice was decreased. This decrease in body weight gain was prevented in As+SLE group mice that received SLE supplemented feed. Arsenic showed a secondary effect by causing enlargement of spleen, kidney and liver of 'As' group mice and this enlargement of the organs was minimized with SLE supplementation. In addition, SLE abrogated arsenic-mediated elevation of serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), uric acid and glucose. These results, therefore, suggest that SLE might have future therapeutic value for preventing or reducing arsenic-induced toxic effects.
由于地下水中存在砷并将其毒性传播给数百万人，砷在孟加拉国造成了严重的公共卫生问题。管理这一受影响人群的治疗选择稀缺，使情况变得更糟。为了找到一种有前景的治疗方法，本研究旨在探讨叶提取物（SLE）对小鼠砷诱导毒性作用的改善作用。将瑞士白化小鼠分为四组，“对照组”接受纯水+正常饲料，“砷（As）组”接受含亚砷酸钠（NaAsO）的水（10μg/g体重/天）+正常饲料，“As+SLE组”接受含NaAsO的水+补充了SLE的饲料（50μg/g体重/天），最后“SLE组”接受纯水+补充了SLE的饲料。在对照小鼠中观察到体重逐渐增加；然而，砷暴露小鼠的体重增加减少。在接受补充了SLE饲料的As+SLE组小鼠中，这种体重增加的减少得到了预防。砷对“As”组小鼠的脾脏、肾脏和肝脏造成肿大，这是一种次要影响，补充SLE可使这些器官的肿大最小化。此外，SLE消除了砷介导的血清碱性磷酸酶（ALP）和谷丙转氨酶（ALT）、乳酸脱氢酶（LDH）、尿酸和葡萄糖的升高。因此，这些结果表明，SLE可能对预防或减少砷诱导的毒性作用具有未来的治疗价值。
