Trusek-Holownia Anna, Noworyta Andrzej
Wroclaw University of Technology, Department of Chemistry, Group of Bioprocess and Biomedical Engineering, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
Biotechnol Rep (Amst). 2015 Jan 9;6:13-19. doi: 10.1016/j.btre.2014.12.012. eCollection 2015 Jun.
The size of the gel preparation, the concentration of the encapsulated enzyme and the ratio of the preparation volume to the volume of the reaction mixture influence the reaction efficiency with encapsulated biocatalysts. A model of first order enzymatic reaction with substrate diffusion is presented and validated by the decomposition reaction of hydrogen peroxide by catalase. The Thiele modulus (Ф) contains the modified (including the enzyme concentration) enzymatic reaction constant ('). Based on the model analysis, the Thiele modulus should not exceed a value of 2 (optimally less than 0.5). This value can be controlled by appropriate selection of the enzyme concentration inside and the size of the capsule. A lower Ф value gives a flat substrate concentration profile inside the gel capsule and all the enzyme molecules are involved in the reaction. The optimal diameter of the gel capsule with respect to their separation from the reaction mixture is 1-2 mm.
凝胶制剂的大小、包封酶的浓度以及制剂体积与反应混合物体积的比例会影响包封生物催化剂的反应效率。本文提出了一个考虑底物扩散的一级酶促反应模型，并通过过氧化氢被过氧化氢酶分解的反应进行了验证。西勒模数（Ф）包含修正后的（包括酶浓度）酶促反应常数（'）。基于模型分析，西勒模数不应超过2（最佳值小于0.5）。该值可通过适当选择内部酶浓度和胶囊大小来控制。较低的Ф值会使凝胶胶囊内部的底物浓度分布较为平缓，且所有酶分子都参与反应。凝胶胶囊相对于与反应混合物分离的最佳直径为1 - 2毫米。
