Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran.
Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:216-220. doi: 10.1016/j.msec.2017.05.044. Epub 2017 May 10.
Described here a methodology for arraying of magnetic nanoparticles (MNPs) on the surface of DNA nanotubes (DNTs). Positioning of magnetic nanoparticles at exterior surface of DNTs were shaped after self-assembling of oligonucleotide staples within an M13mp18 DNA scaffold via an origami process. The staples were partially labeled with biotin to be arrayed at the surface of DNTs. Gel retardation assay of the DNTs carrying magnetic nanoparticles indicated a reversely behavioral electrophoretic movement in comparison to the nanotubes have been demonstrated previously. Also, high resolution transmission electron microscopy confirmed positioning magnetic nanoparticles at the exterior surface of DNTs, correctly. Ultrastructural characteristics of these DNA nanotubes using atomic force microscopy demonstrated topographic heights on their surfaces formed through positioning of magnetic nanoparticles outside the tubules. This nanoarchitecture would be potential for multiple arraying of nanoparticles that those be useful as functionalized chimeric nanocarriers for developing novel nanodrugs and nanobiosensors.
这里描述了一种在 DNA 纳米管（DNTs）表面排列磁性纳米颗粒（MNPs）的方法。通过折纸工艺，在 M13mp18 DNA 支架内寡核苷酸订书钉自组装后，将磁性纳米颗粒定位在 DNTs 的外表面。订书钉部分用生物素标记，以便在 DNTs 表面排列。携带磁性纳米颗粒的 DNTs 的凝胶阻滞实验表明，与之前已经证明的纳米管相比，它们表现出反向电泳行为。此外，高分辨率透射电子显微镜也证实了磁性纳米颗粒正确地位于 DNTs 的外表面。使用原子力显微镜对这些 DNA 纳米管进行超微结构特征研究表明，通过将磁性纳米颗粒定位在管外，在其表面形成了拓扑高度。这种纳米结构可以进行多个纳米颗粒的排列，这些纳米颗粒可用作功能化嵌合纳米载体，用于开发新型纳米药物和纳米生物传感器。
