问答：扩展显微镜技术

Gao Ruixuan, Asano Shoh M, Boyden Edward S

Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.

Internal Medicine Research Unit, Pfizer Inc., Cambridge, MA, USA.

BMC Biol. 2017 Jun 19;15(1):50. doi: 10.1186/s12915-017-0393-3.

Expansion microscopy (ExM) is a recently invented technology that uses swellable charged polymers, synthesized densely and with appropriate topology throughout a preserved biological specimen, to physically magnify the specimen 100-fold in volume, or more, in an isotropic fashion. ExM enables nanoscale resolution imaging of preserved samples on inexpensive, fast, conventional microscopes. How does ExM work? How good is its performance? How do you get going on using it? In this Q&A, we provide the answers to these and other questions about this new and rapidly spreading toolbox.

扩展显微镜技术（ExM）是一项最近发明的技术，它使用可膨胀的带电聚合物，在整个保存的生物样本中密集合成并具有适当的拓扑结构，以各向同性的方式将样本的体积物理放大100倍或更多。ExM能够在廉价、快速的传统显微镜上对保存的样本进行纳米级分辨率成像。ExM是如何工作的？它的性能如何？如何开始使用它？在这个问答中，我们提供了关于这个新的且迅速传播的工具包的这些问题及其他问题的答案。