Lee Hyo-Jin, Whang Kyung-Sook
Institute of Microbial Ecology & Resources, Mokwon University, Daejeon 302-729, Republic of Korea.
Department of Microbial & Nano Materials, Mokwon University, Daejeon 302-729, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1746-1751. doi: 10.1099/ijsem.0.001858. Epub 2017 Jun 20.
A novel actinobacterium, designated strain UDF-1T, was isolated from forest soil in Chungnam, South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain UDF-1T formed a branched brownish-orange substrate mycelium with spherical or oval spores. No aerial mycelium was formed. Comparative 16S rRNA gene sequence analysis indicated that strain UDF-1T belongs to the genus Micromonospora, showing the highest sequence similarity to Micromonospora palomenae NEAU-CX1T (99.2 % 16S rRNA gene sequence similarity), 'Micromonospora maoerensis' NEAU-MES19 (99.0 %), Micromonospora endolithica DSM 44398T (98.8 %) and Micromonospora matsumotoense IMSNU 22003T (98.8 %). The predominant menaquinones of strain UDF-1T were MK-10 (H4) and MK-10 (H6). The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were arabinose and xylose. The major polar lipids were phosphatidylinositol, diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The genomic DNA G+C content was 73.1 mol%. DNA-DNA relatedness between strain UDF-1T and closely related type strains in the genus Micromonospora was below 30 %. On the basis of the polyphasic analysis conducted in this study, strain UDF-1T represents a novel species of the genus Micromonospora, for which the name Micromonospora fulva sp. nov. is proposed. The type strain is UDF-1T (=KACC 18696T=NBRC 111826T).
从韩国忠南的森林土壤中分离出一株新的放线菌，命名为菌株UDF-1T，并采用多相分类法对其分类地位进行了研究。菌株UDF-1T形成具分支的棕橙色基内菌丝体，带有球形或椭圆形孢子，未形成气生菌丝体。16S rRNA基因序列比较分析表明，菌株UDF-1T属于小单孢菌属，与帕洛米小单孢菌NEAU-CX1T（16S rRNA基因序列相似性为99.2%）、“茂尔小单孢菌”NEAU-MES19（99.0%）、石内小单孢菌DSM 44398T（98.8%）和松本小单孢菌IMSNU 22003T（98.8%）显示出最高的序列相似性。菌株UDF-1T的主要甲基萘醌为MK-10（H4）和MK-10（H6）。细胞壁含有内消旋二氨基庚二酸，全细胞糖为阿拉伯糖和木糖。主要极性脂为磷脂酰肌醇、双磷脂酰甘油和磷脂酰乙醇胺。主要细胞脂肪酸为异-C16 : 0、anteiso-C15 : 0和异-C15 : 0。基因组DNA G+C含量为73.1 mol%。菌株UDF-1T与小单孢菌属密切相关模式菌株之间的DNA-DNA相关性低于30%。基于本研究进行的多相分析，菌株UDF-1T代表小单孢菌属的一个新物种，为此提出新名称微黄小单孢菌（Micromonospora fulva sp. nov.）。模式菌株为UDF-1T（=KACC 18696T=NBRC 111826T）。
