Xu Zhen-Xing, Zhang Heng-Xi, Han Ji-Ru, Dunlap Christopher A, Rooney Alejandro P, Mu Da-Shuai, Du Zong-Jun
College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1969-1974. doi: 10.1099/ijsem.0.001897. Epub 2017 Jun 20.
A novel Gram-stain-negative, facultatively anaerobic, yellowish and agar-digesting marine bacterium, designated strain QM50T, was isolated from coastal seawater in an aquaculture site near Qingdao, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represented a member of the genus Colwellia and exhibited the highest sequence similarity (97.4 %) to Colwellia aestuarii SMK-10T. Average nucleotide identity (ANI) values based on draft genome sequences between strain QM50T and C. aestuarii KCTC 12480T showed a relatedness of 72.0 % (ANIb) and 85.1 % (ANIm). Cells of strain QM50T were approximately 0.3-0.6×0.8-2.5 µm in size and motile by means of a polar flagellum. Growth occurred in the presence of 1.0-6.0 % (w/v) NaCl (optimum, 2.0-3.0 %), at pH 6.5-8.5 (optimum, pH 7.0) and at 4-37 °C (optimum, 28-30 °C). Strain QM50T was found to contain ubiquinone 8 (Q-8) as the predominant ubiquinone and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and C17 : 1ω8c as the main cellular fatty acids. Phosphatidylethanolamine and phosphatidylglycerol were found to be major polar lipids. The DNA G+C content of strain QM50T was determined to be 35.7 mol%. On the basis of phylogenetic and phenotypic data, strain QM50T represents a novel species of the genus Colwellia, for which the name Colwellia agarivorans sp. nov. is proposed. The type strain is QM50T (=KCTC 52273T=MCCC 1H00143T).
从中国青岛附近一个水产养殖场地的沿海海水中分离出一株新型革兰氏阴性、兼性厌氧、呈黄色且能消化琼脂的海洋细菌，命名为菌株QM50T。基于16S rRNA基因序列的系统发育分析表明，该分离株属于科尔韦氏菌属，与河口科尔韦氏菌SMK-10T的序列相似性最高（97.4%）。基于草图基因组序列的菌株QM50T与河口科尔韦氏菌KCTC 12480T之间的平均核苷酸同一性（ANI）值显示，两者的相关性为72.0%（ANIb）和85.1%（ANIm）。菌株QM50T的细胞大小约为0.3 - 0.6×0.8 - 2.5 µm，通过极生鞭毛运动。在1.0 - 6.0%（w/v）NaCl（最适浓度为2.0 - 3.0%）、pH 6.5 - 8.5（最适pH为7.0）和4 - 37 °C（最适温度为28 - 30 °C）条件下生长。发现菌株QM50T以泛醌8（Q-8）作为主要泛醌，以总和特征3（C16 : 1ω7c和/或异C15 : 0 2-OH）、C16 : 0和C17 : 1ω8c作为主要细胞脂肪酸。发现磷脂酰乙醇胺和磷脂酰甘油是主要极性脂质。菌株QM50T的DNA G+C含量测定为35.7 mol%。基于系统发育和表型数据，菌株QM50T代表科尔韦氏菌属的一个新物种，为此提出新名称琼脂食科尔韦氏菌（Colwellia agarivorans sp. nov.）。模式菌株为QM50T（=KCTC 52273T=MCCC 1H00143T）
