活体小鼠和人类中的斑点调制光学相干断层扫描。
Speckle-modulating optical coherence tomography in living mice and humans.
机构信息
Department of Structural Biology, Stanford University, Stanford, California 94305, USA.
Department of Electrical Engineering, Stanford University, Stanford, California 94305, USA.
出版信息
Nat Commun. 2017 Jun 20;8:15845. doi: 10.1038/ncomms15845.
Optical coherence tomography (OCT) is a powerful biomedical imaging technology that relies on the coherent detection of backscattered light to image tissue morphology in vivo. As a consequence, OCT is susceptible to coherent noise (speckle noise), which imposes significant limitations on its diagnostic capabilities. Here we show speckle-modulating OCT (SM-OCT), a method based purely on light manipulation that virtually eliminates speckle noise originating from a sample. SM-OCT accomplishes this by creating and averaging an unlimited number of scans with uncorrelated speckle patterns without compromising spatial resolution. Using SM-OCT, we reveal small structures in the tissues of living animals, such as the inner stromal structure of a live mouse cornea, the fine structures inside the mouse pinna, and sweat ducts and Meissner's corpuscle in the human fingertip skin-features that are otherwise obscured by speckle noise when using conventional OCT or OCT with current state of the art speckle reduction methods.
光学相干断层扫描（OCT）是一种强大的生物医学成像技术，它依赖于背向散射光的相干检测来对体内组织形态进行成像。因此，OCT 容易受到相干噪声（散斑噪声）的影响，这对其诊断能力有很大的限制。在这里，我们展示了基于纯光操控的散斑调制光学相干断层扫描（SM-OCT）方法，该方法可以有效地消除来自样本的散斑噪声。SM-OCT 通过创建和平均具有不相关散斑图案的无限数量的扫描来实现这一点，而不会牺牲空间分辨率。使用 SM-OCT，我们揭示了活体动物组织中的小结构，例如活体小鼠角膜的内部基质结构、小鼠耳廓内部的精细结构以及人指尖皮肤中的汗管和 Meissner 小体——这些结构在使用传统 OCT 或具有当前最先进的散斑减少方法的 OCT 时，会被散斑噪声掩盖。
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