Zhou Kevin C, McNabb Ryan P, Qian Ruobing, Degan Simone, Dhalla Al-Hafeez, Farsiu Sina, Izatt Joseph A
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710, USA.
Optica. 2022 Jun 20;9(6):593-601. doi: 10.1364/optica.454860. Epub 2022 Jun 2.
Optical coherence tomography (OCT) has seen widespread success as an in vivo clinical diagnostic 3D imaging modality, impacting areas including ophthalmology, cardiology, and gastroenterology. Despite its many advantages, such as high sensitivity, speed, and depth penetration, OCT suffers from several shortcomings that ultimately limit its utility as a 3D microscopy tool, such as its pervasive coherent speckle noise and poor lateral resolution required to maintain millimeter-scale imaging depths. Here, we present 3D optical coherence refraction tomography (OCRT), a computational extension of OCT which synthesizes an incoherent contrast mechanism by combining multiple OCT volumes, acquired across two rotation axes, to form a resolution-enhanced, speckle-reduced, refraction-corrected 3D reconstruction. Our label-free computational 3D microscope features a novel optical design incorporating a parabolic mirror to enable the capture of 5D plenoptic datasets, consisting of millimetric 3D fields of view over up to ±75° without moving the sample. We demonstrate that 3D OCRT reveals 3D features unobserved by conventional OCT in fruit fly, zebrafish, and mouse samples.
光学相干断层扫描（OCT）作为一种体内临床诊断三维成像方式已取得广泛成功，其影响领域包括眼科、心脏病学和胃肠病学。尽管OCT具有诸多优点，如高灵敏度、速度快和深度穿透能力强，但它也存在一些缺点，这些缺点最终限制了其作为三维显微镜工具的实用性，例如普遍存在的相干散斑噪声以及为保持毫米级成像深度而需要的较差横向分辨率。在此，我们展示了三维光学相干折射断层扫描（OCRT），这是OCT的一种计算扩展，它通过组合在两个旋转轴上采集的多个OCT体积来合成非相干对比机制，以形成分辨率增强、散斑减少且经过折射校正的三维重建。我们的无标记计算三维显微镜具有一种新颖的光学设计，其中包含一个抛物面镜，能够在不移动样品的情况下捕获五维全光数据集，该数据集由高达±75°范围内的毫米级三维视场组成。我们证明，三维OCRT揭示了果蝇、斑马鱼和小鼠样本中传统OCT未观察到的三维特征。
