Rogers M J, Appella E, Pierotti M A, Invernizzi G, Parmiani G
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1415-9. doi: 10.1073/pnas.76.3.1415.
A methylcholanthrene-induced tumor of BALB/c (H-2d) origin had been shown to express H-2 antigens normally associated with the H-2k haplotype. The molecules expressing the alien antigens have been partially purified and shown to be distinct from molecules expressing normal H-2d antigens. Like normal H-2 antigens, the alien antigens have a molecular weight of 48,000, are glycoproteins, and are noncovalently bound to beta 2-microglobulin. The alien antigens differ from normal H-2 antigens in their susceptibility to papain digestion, behavior during gel filtration, and overall stability. Possible mechanisms accounting for the expression of the alien antigen are discussed.
已证明源自BALB/c(H-2d)的甲基胆蒽诱导肿瘤正常表达通常与H-2k单倍型相关的H-2抗原。表达外来抗原的分子已被部分纯化,并显示与表达正常H-2d抗原的分子不同。与正常H-2抗原一样,外来抗原的分子量为48,000,是糖蛋白,并且与β2-微球蛋白非共价结合。外来抗原在对木瓜蛋白酶消化的敏感性、凝胶过滤过程中的行为以及整体稳定性方面与正常H-2抗原不同。讨论了解释外来抗原表达的可能机制。