Kaur Manjeet, Kendre Shrikant, Gupta Parmod, Singh Navneet, Sethi Harsimran, Gupta Neha, Acharya Rushil
Maharaja Ganga Singh Dental College and Research Centre 11LNT Hanumangarh Road, Shri Ganganagar, Rajasthan.
J Clin Exp Dent. 2017 Jun 1;9(6):e799-e804. doi: 10.4317/jced.53053. eCollection 2017 Jun.
is a microorganism commonly detected in asymptomatic, persistent endodontic infections. Triple antibiotic paste has stood the test of time as a proven antibiotic combination against . However, problems with this include staining of teeth and standardization of the preparation. Thus, the search for better alternatives and better preparation techniques is still on.
To observe the potential of combinations [(Amoxicillin+ Metronidazole, Amoxicillin Clavulanic Acid + Metronidazole; Amoxicillin and Cloxacillin + Metronidazole)] over Triple Antibiotic Paste.
Fifty single rooted teeth free from dental caries were selected for the study. Teeth were cut at equal distance from root apex (13mm from apex) with sterile diamond disk and straight hand piece for standardization of root length. The opening of root canal was enlarged with engine driven pro-taper files. To remove the organic and inorganic debris, canal was cleaned with 17% EDTA followed by 2.5% NaOCl for 5min. Distilled water irrigation was done for 5 min to remove any traces of used chemical and then sterilized in autoclave at 1200c for 15 min. at 15 lbs pressure. Bacteria cultured on blood agar plate and at the same time fresh antibiotic combinations were made and placed in the root canals, then incubated in the incubator, under sterile conditions and observed at 24hrs, 48hr and 72hrs.
The largest inhibition zones were observed for the Triple Antibiotic Paste, followed by Amoxicillin and Clavulanic acid + Metronidazole group however, the clearest zones were achieved with Amoxicillin and Clavlunic acid + Metronidazole group and the smallest for Amoxicillin and Metronidazole group.
The results suggest that though Triple antibiotic showed the maximum inhibition, Amoxicillin and Clavulanic acid combination along with Metronidazole gave the most reliable results. Further studies using the different combinations and different concentrations along with different methods of increasing the shelf life of such medications can be undertaken. Enterococcus faecalis, Triple Antibiotic Paste, Amoxcillin, Clavulanic acid.
粪肠球菌是在无症状、持续性牙髓感染中常见检测到的一种微生物。三联抗生素糊剂作为一种经证实的针对粪肠球菌的抗生素组合，经受住了时间的考验。然而，其存在牙齿染色和制剂标准化等问题。因此，对更好的替代方案和更好的制备技术的探索仍在继续。
观察（阿莫西林 + 甲硝唑、阿莫西林克拉维酸 + 甲硝唑、阿莫西林和氯唑西林 + 甲硝唑）组合相对于三联抗生素糊剂的潜力。
选择50颗无龋的单根牙进行研究。用无菌金刚石盘和直手机在距根尖等距离（距根尖13mm）处切割牙齿，以使根长标准化。用机用ProTaper锉扩大根管口。为去除有机和无机碎屑，先用17%乙二胺四乙酸（EDTA）冲洗根管，再用2.5%次氯酸钠（NaOCl）冲洗5分钟。用蒸馏水冲洗5分钟以去除任何残留的用过的化学物质，然后在120℃、15磅压力下在高压灭菌器中灭菌15分钟。在血琼脂平板上培养细菌，同时制备新鲜的抗生素组合并置于根管中，然后在无菌条件下在培养箱中孵育，并在24小时、48小时和72小时观察。
三联抗生素糊剂观察到的抑菌圈最大，其次是阿莫西林和克拉维酸 + 甲硝唑组，然而，阿莫西林和克拉维酸 + 甲硝唑组的抑菌圈最清晰，阿莫西林和甲硝唑组的抑菌圈最小。
结果表明，虽然三联抗生素显示出最大的抑菌作用，但阿莫西林和克拉维酸组合与甲硝唑一起产生了最可靠的结果。可以进行进一步的研究，使用不同的组合、不同的浓度以及不同的延长此类药物保质期的方法。粪肠球菌、三联抗生素糊剂、阿莫西林、克拉维酸。
