单细胞中抗癌药物作用的成像
Imaging of anticancer drug action in single cells.
作者信息
Miller Miles A, Weissleder Ralph
机构信息
Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
出版信息
Nat Rev Cancer. 2017 Jun 23;17(7):399-414. doi: 10.1038/nrc.2017.41.
Abstract
Imaging is widely used in anticancer drug development, typically for whole-body tracking of labelled drugs to different organs or to assess drug efficacy through volumetric measurements. However, increasing attention has been drawn to pharmacology at the single-cell level. Diverse cell types, including cancer-associated immune cells, physicochemical features of the tumour microenvironment and heterogeneous cell behaviour all affect drug delivery, response and resistance. This Review summarizes developments in the imaging of in vivo anticancer drug action, with a focus on microscopy approaches at the single-cell level and translational lessons for the clinic.
摘要
成像技术在抗癌药物研发中被广泛应用，通常用于对标记药物在全身向不同器官的追踪，或通过体积测量来评估药物疗效。然而，单细胞水平的药理学研究已越来越受到关注。多种细胞类型，包括癌症相关免疫细胞、肿瘤微环境的物理化学特征以及异质性细胞行为，都会影响药物递送、反应和耐药性。本综述总结了体内抗癌药物作用成像的进展，重点关注单细胞水平的显微镜方法以及对临床的转化经验。
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