Amadi Victor A, Hariharan Harry, Amadi Ozioma A, Matthew-Belmar Vanessa, Nicholas-Thomas Roxanne, Perea Marta Lanza, Carter Kenrith, Rennie Eugene, Kalasi Keith, Alhassan Andy, Kabuusu Richard M, Alozie Grant Ugochukwu, Fields Paul J, Pinckney Rhonda, Sharma Ravindra
Department of Pathobiology, School of Veterinary Medicine, St. George's University, St. George's, Grenada, West Indies.
Department of Public Health and Preventative Medicine, School of Medicine, St. George's University, Grenada, West Indies.
Vet World. 2019 Dec;12(12):2070-2075. doi: 10.14202/vetworld.2019.2070-2075. Epub 2019 Dec 27.
There is currently no published information on the prevalence and antimicrobial susceptibility patterns of commensal in dogs of Grenada origin. Monitoring antimicrobial resistance helps in the empirical selection of antibiotics. This study determined the occurrence of including the O157:H7 serotype in feces of non-diarrheic dogs of Grenada origin and the antibiotic resistance pattern of the isolates.
Fecal samples from 142 of the 144 (98.6%) dogs were culture positive for . Selection of up to three colonies from each of the 142 -positive samples yielded a total of 402 isolates, which were analyzed for the presence of non-sorbitol fermenting colonies, and O157-agglutination.
Of the 402 isolates, 30 (7.5%) were non-sorbitol fermenters. However, none of the 402 isolates gave a positive reaction (O157:H7) to the O157:H7 latex kit. Antimicrobial susceptibility tests against 12 antibiotics revealed low resistance rates to all the tested antibiotics except for tetracycline (Te) (23.4%), cephalothin (CF) (13.2%), and ampicillin (AM) (7.7%). Thirty-nine out of the 402 (9.7%), isolates were resistant to two or more antibiotics of different classes.
This is the first report of isolation and antimicrobial susceptibilities of commensal from non-diarrheic dogs in Grenada. Some of the isolates (39/402 isolates, 9.7%) were resistant to multiple antibiotics. This study showed that presently, dogs in Grenada should not be considered a reservoir for the O157:H7 serotype and for multiple antibiotic-resistant strains. Among the 402 isolates, the resistance rate to drugs other than Te, CF, and AM was very low.
目前尚无关于格林纳达原产犬共生大肠杆菌的流行情况及抗菌药物敏感性模式的公开信息。监测抗菌药物耐药性有助于经验性选择抗生素。本研究确定了格林纳达原产非腹泻犬粪便中包括O157:H7血清型在内的大肠杆菌的发生情况以及大肠杆菌分离株的抗生素耐药模式。
144只犬中的142只（98.6%）粪便样本经培养后大肠杆菌呈阳性。从142份大肠杆菌阳性样本中每份最多选取3个菌落，共获得402株大肠杆菌分离株，对其进行非山梨醇发酵菌落和O157凝集试验分析。
在402株大肠杆菌分离株中，30株（7.5%）为非山梨醇发酵菌。然而，402株分离株中没有一株对O157:H7乳胶试剂盒产生阳性反应（O157:H7）。对12种抗生素的药敏试验显示，除四环素（Te）（23.4%）、头孢噻吩（CF）（13.2%）和氨苄西林（AM）（7.7%）外，所有受试抗生素的耐药率都很低。402株（9.7%）大肠杆菌分离株中有39株对两种或更多不同类别的抗生素耐药。
这是关于格林纳达非腹泻犬共生大肠杆菌分离及药敏情况的首次报告。部分分离株（39/402株，9.7%）对多种抗生素耐药。本研究表明，目前格林纳达的犬不应被视为O157:H7血清型和多重耐药大肠杆菌菌株的储存宿主。在402株大肠杆菌分离株中，除Te、CF和AM外，对其他药物的耐药率非常低。
