Ellner P D, Williams D A, Hosmer M E, Cohenford M A
J Clin Microbiol. 1985 Nov;22(5):880-1. doi: 10.1128/jcm.22.5.880-881.1985.
A rapid colorimetric method for the presumptive identification of group A streptococci and enterococci based upon pyroglutamyl aminopeptidase activity is described. Of 76 group A streptococcal isolates from primary plates, 83 gave positive reactions, and the remaining 7 were positive on retesting in pure culture. Of the 31 enterococcal isolates tested, all gave positive reactions. Despite occasional positive reactions with staphylococci and Klebsiella pneumoniae, the test could be useful and cost-effective in the clinical laboratory.
描述了一种基于焦谷氨酸氨基肽酶活性的用于初步鉴定A群链球菌和肠球菌的快速比色法。从原始平板分离出的76株A群链球菌中,83株呈阳性反应,其余7株在纯培养物中重新检测时呈阳性。在检测的31株肠球菌分离物中,全部呈阳性反应。尽管偶尔葡萄球菌和肺炎克雷伯菌会出现阳性反应,但该试验在临床实验室可能有用且具有成本效益。