用于快速鉴定肠球菌的氯化钠-七叶苷水解试验
Sodium chloride-esculin hydrolysis test for rapid identification of enterococci.
作者信息
Qadri S M, Flournoy D J, Qadri S G
出版信息
J Clin Microbiol. 1987 Jun;25(6):1107-8. doi: 10.1128/jcm.25.6.1107-1108.1987.
Abstract
The ability of enterococci to cause severe disease in humans and their relative resistance to chemotherapeutic agents make it desirable to rapidly differentiate these organisms from other streptococci. We developed and evaluated a test that within 2 h distinguishes enterococci from other alpha-, beta-, or nonhemolytic streptococci in a buffered solution containing 0.2% esculin and 5% sodium chloride. All 239 strains of enterococci tested gave a positive reaction within 2 h, whereas 95 of 96 isolates of other streptococci remained negative at 4 h.
摘要
肠球菌在人类中引发严重疾病的能力以及它们对化疗药物的相对耐药性，使得快速将这些微生物与其他链球菌区分开来变得很有必要。我们开发并评估了一种检测方法，该方法能在2小时内，在含有0.2%七叶苷和5%氯化钠的缓冲溶液中，将肠球菌与其他α-、β-或非溶血性链球菌区分开来。所有测试的239株肠球菌在2小时内均呈阳性反应，而96株其他链球菌分离株中的95株在4小时时仍为阴性。
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