Alas Guillermo R, Agarwal Rachit, Collard David M, García Andrés J
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Acta Biomater. 2017 Sep 1;59:108-116. doi: 10.1016/j.actbio.2017.06.033. Epub 2017 Jun 24.
The modification of the surface of surgical implants with cell adhesion ligands has emerged as a promising approach to improve biomaterial-host interactions. However, these approaches are limited by the non-specific adsorption of biomolecules and uncontrolled presentation of desired bioactive ligands on implant surfaces. This leads to sub-optimal integration with host tissue and delayed healing. Here we present a strategy to grow non-fouling polymer brushes of oligo(ethylene glycol) methacrylate by atom transfer radical polymerization from dopamine-functionalized clinical grade 316 stainless steel. These brushes prevent non-specific adsorption of proteins and attachment of cells. Subsequently, the brushes can be modified with covalently tethered adhesive peptides that provide controlled cell adhesion. This approach may therefore have broad application to promote bone growth and improvements in osseointegration.
Stainless steel (SS) implants are widely used clinically for orthopaedic, spinal, dental and cardiovascular applications. However, non-specific adsorption of biomolecules onto implant surfaces results in sub-optimal integration with host tissue. To allow controlled cell-SS interactions, we have developed a strategy to grow non-fouling polymer brushes that prevent protein adsorption and cell adhesion and can be subsequently functionalized with adhesive peptides to direct cell adhesion and signaling. This approach has broad application to improve osseointegration onto stainless steel implants in bone repair.
用细胞黏附配体修饰外科植入物表面已成为一种改善生物材料与宿主相互作用的有前景的方法。然而，这些方法受到生物分子非特异性吸附以及植入物表面所需生物活性配体呈现不受控制的限制。这导致与宿主组织的整合不理想以及愈合延迟。在此，我们提出一种策略，通过原子转移自由基聚合从多巴胺功能化的临床级316不锈钢上生长聚甲基丙烯酸寡聚乙二醇的抗污聚合物刷。这些刷可防止蛋白质的非特异性吸附和细胞附着。随后，这些刷可用共价连接的黏附肽进行修饰，从而实现可控的细胞黏附。因此，这种方法可能在促进骨生长和改善骨整合方面有广泛应用。
不锈钢（SS）植入物在临床上广泛用于骨科、脊柱、牙科和心血管应用。然而，生物分子在植入物表面的非特异性吸附导致与宿主组织的整合不理想。为了实现可控的细胞与不锈钢相互作用，我们开发了一种策略来生长抗污聚合物刷，这种刷可防止蛋白质吸附和细胞黏附，随后可用黏附肽进行功能化修饰以引导细胞黏附和信号传导。这种方法在改善骨修复中不锈钢植入物的骨整合方面有广泛应用。
