Kubota Y, Watanabe Y, Otsuka H, Tamiya T, Tsuchiya T, Matsumoto J J
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;82(2):345-8. doi: 10.1016/0742-8413(85)90173-2.
The antibacterial factor from the body surface of the African giant snail, Achatina fulica Férussac, was isolated by DEAE-Toyopearl 650M ion exchange chromatography. The isolated preparation exhibited highly positive antibacterial activity both for the Gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus and for the Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, but it lost such activity when heated at 75 degrees C for 5 min. The antibacterial factor of the snail mucus was a glycoprotein whose molecular weight (MW) was about 160,000. It was composed of two subunits of MW 70,000-80,000.
通过DEAE- Toyopearl 650M离子交换色谱法从非洲大蜗牛（Achatina fulica Férussac）体表分离出抗菌因子。分离得到的制剂对革兰氏阳性菌枯草芽孢杆菌和金黄色葡萄球菌以及革兰氏阴性菌大肠杆菌和铜绿假单胞菌均表现出高度阳性的抗菌活性，但在75℃加热5分钟后失去该活性。蜗牛黏液的抗菌因子是一种糖蛋白，其分子量约为160,000。它由两个分子量为70,000 - 80,000的亚基组成。
