Chen Zhaojun, Yu Daojun, He Songzhe, Ye Hui, Zhang Lei, Wen Yanping, Zhang Wenhui, Shu Liping, Chen Shuchang
Department of Environmental and Occupational Health, Hangzhou Center for Disease Control and PreventionHangzhou, China.
Department of Clinical Laboratory, Hangzhou First People's HospitalHangzhou, China.
Front Microbiol. 2017 Jun 16;8:1133. doi: 10.3389/fmicb.2017.01133. eCollection 2017.
This study investigated the distribution of antibiotic resistant () and examined the possible relationship between water quality parameters and antibiotic resistance from two different drinking water sources (the Qiantang River and the Dongtiao Stream) in Hangzhou city of China. isolates were tested for their susceptibility to 18 antibiotics. Most of the isolates were resistant to tetracycline (TE), followed by ampicillin (AM), piperacillin (PIP), trimethoprim/sulfamethoxazole (SXT), and chloramphenicol (C). The antibiotic resistance rate of isolates from two water sources was similar; For isolates from the Qiantang River, their antibiotic resistance rates decreased from up- to downstream. Seasonally, the dry and wet season had little impact on antibiotic resistance. Spearman's rank correlation revealed significant correlation between resistance to TE and phenicols or ciprofloxacin (CIP), as well as quinolones (ciprofloxacin and levofloxacin) and cephalosporins or gentamicin (GM). Pearson's chi-square tests found certain water parameters such as nutrient concentration were strongly associated with resistance to some of the antibiotics. In addition, genes were detected from all 82 TE-resistant isolates, and most of the isolates (81.87%) contained multiple genes, which displayed 14 different combinations. Collectively, this study provided baseline data on antibiotic resistance of drinking water sources in Hangzhou city, which indicates drinking water sources could be the reservoir of antibiotic resistance, potentially presenting a public health risk.
本研究调查了抗生素抗性细菌的分布情况，并考察了中国杭州市两个不同饮用水源（钱塘江和东苕溪）的水质参数与抗生素抗性之间的可能关系。对分离出的细菌进行了18种抗生素的敏感性测试。大多数分离菌株对四环素（TE）耐药，其次是氨苄西林（AM）、哌拉西林（PIP）、甲氧苄啶/磺胺甲恶唑（SXT）和氯霉素（C）。两个水源分离菌株的抗生素耐药率相似；对于钱塘江分离出的菌株，其抗生素耐药率从上游到下游呈下降趋势。季节性方面，旱季和雨季对抗生素耐药性影响不大。Spearman秩相关分析显示，对TE的耐药性与对酚类或环丙沙星（CIP）的耐药性之间存在显著相关性，以及喹诺酮类（环丙沙星和左氧氟沙星）与头孢菌素类或庆大霉素（GM）之间也存在显著相关性。Pearson卡方检验发现某些水质参数，如营养物浓度，与对某些抗生素的耐药性密切相关。此外，在所有82株对TE耐药的细菌分离物中均检测到了 基因，并且大多数分离物（81.87%）含有多个 基因，这些基因呈现出14种不同的组合。总体而言，本研究提供了杭州市饮用水源抗生素抗性的基线数据，表明饮用水源可能是抗生素抗性的储存库， potentially presenting a public health risk.（最后一句“potentially presenting a public health risk”在原文中位置较奇怪，推测可能是想表达“这可能会带来公共健康风险”，但按照要求未做修改）可能会带来公共健康风险。
