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在新生小鼠药物诱导的类似运动活动期间，运动神经元调节中枢模式发生器。

Motoneurons regulate the central pattern generator during drug-induced locomotor-like activity in the neonatal mouse.

作者信息

Falgairolle Melanie, Puhl Joshua G, Pujala Avinash, Liu Wenfang, O'Donovan Michael J

机构信息

Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States.

Department of Entomology, University of Minnesota, Saint Paul, United States.

出版信息

Elife. 2017 Jul 3;6:e26622. doi: 10.7554/eLife.26622.

DOI:10.7554/eLife.26622
PMID:28671548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5550280/
Abstract

Motoneurons are traditionally viewed as the output of the spinal cord that do not influence locomotor rhythmogenesis. We assessed the role of motoneuron firing during ongoing locomotor-like activity in neonatal mice expressing archaerhopsin-3 (Arch), halorhodopsin (eNpHR), or channelrhodopsin-2 (ChR2) in Choline acetyltransferase neurons (ChAT) or Arch in LIM-homeodomain transcription factor neurons. Illumination of the lumbar cord in mice expressing eNpHR or Arch in ChAT or neurons, depressed motoneuron discharge, transiently decreased the frequency, and perturbed the phasing of the locomotor-like rhythm. When the light was turned off motoneuron firing and locomotor frequency both transiently increased. These effects were not due to cholinergic neurotransmission, persisted during partial blockade of gap junctions and were mediated, in part, by AMPAergic transmission. In spinal cords expressing ChR2, illumination increased motoneuron discharge and transiently accelerated the rhythm. We conclude that motoneurons provide feedback to the central pattern generator (CPG) during drug-induced locomotor-like activity.

摘要

传统观点认为运动神经元是脊髓的输出部分，不影响运动节律的产生。我们评估了在胆碱乙酰转移酶神经元（ChAT）中表达古菌视紫红质-3（Arch）、嗜盐视紫红质（eNpHR）或通道视紫红质-2（ChR2）的新生小鼠，或在LIM同源域转录因子神经元中表达Arch的小鼠，在进行类似运动的活动期间运动神经元放电的作用。在ChAT或神经元中表达eNpHR或Arch的小鼠，对腰髓进行光照，会抑制运动神经元放电，短暂降低频率，并扰乱类似运动的节律相位。当光关闭时，运动神经元放电和运动频率都会短暂增加。这些效应不是由于胆碱能神经传递，在间隙连接部分阻断期间持续存在，并且部分由AMPA能传递介导。在表达ChR2的脊髓中，光照增加运动神经元放电并短暂加速节律。我们得出结论，在药物诱导的类似运动的活动期间，运动神经元向中枢模式发生器（CPG）提供反馈。

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