Choi Hyeon-Son, Ko Bong Soo, Kim Hae Dun, Hong Ki-Bae, Suh Hyung Joo
Department of Food Science and Technology, Seoul Women's University.
Research and Development Center, Namyang Dairy Products Corporation.
Biol Pharm Bull. 2017;40(7):1101-1110. doi: 10.1248/bpb.b17-00262.
The aim of this study was to investigate the sleep-promoting effect of a Valerian/Hops mixture in fruit flies. The HPLC analysis showed that Valerenic acid (1260.53 µg/g of extract) and Xanthohumol (Cascade: 827.49 µg/g, Hallertau: 763.60 µg/g, Saaz: 186.93 µg/g) were contained in Valerian and Hop, respectively. The sleep patterns of fruit flies on the Valerian/Hops were examined in both baseline and caffeine-treated conditions. Total activities of flies significantly decreased in 20 mg/mL Valerian (74%), 10 mg/mL Cascade (25%), and 5 mg/mL Hallertau (11%) during nighttime or daytime compared with the control. Valerian/Cascade mixture showed longer sleeping time (ca. 20%) than control group. This mixture-mediated effect was partly observed in caffeine-treated flies. Valerian/Cascade mixture upregulated mRNA expressions of gamma-aminobutyric acid (GABA) receptors and serotonin receptor, and GABA receptors were more strongly regulated than serotonin receptor. In competitive GABA receptor binding assay, Valerian/Cascade mixture extract showed a higher binding ability on GABA receptor than Valerenic acid or/and Xanthohumol which are estimated to be active compounds in the extract. This study demonstrates that a Valerian/Cascade mixture extract improves sleep-related behaviors, including sleeping time, by modulating GABAergic/serotonergic signaling.
本研究旨在调查缬草/啤酒花混合物对果蝇的促睡眠作用。高效液相色谱分析表明，缬草中含有缬草烯酸（1260.53μg/g提取物），啤酒花中分别含有黄腐酚（卡斯卡特：827.49μg/g，哈拉道：763.60μg/g，萨兹：186.93μg/g）。在基线和咖啡因处理条件下，检测了果蝇在缬草/啤酒花上的睡眠模式。与对照组相比，在夜间或白天，20mg/mL缬草（74%）、10mg/mL卡斯卡特（25%）和5mg/mL哈拉道（11%）中的果蝇总活动显著降低。缬草/卡斯卡特混合物的睡眠时间比对照组延长约20%。在咖啡因处理的果蝇中部分观察到了这种混合物介导的效应。缬草/卡斯卡特混合物上调了γ-氨基丁酸（GABA）受体和5-羟色胺受体的mRNA表达，且GABA受体的上调程度比5-羟色胺受体更强。在竞争性GABA受体结合试验中，缬草/卡斯卡特混合物提取物对GABA受体的结合能力高于缬草烯酸或/和黄腐酚，而缬草烯酸和黄腐酚被认为是提取物中的活性化合物。本研究表明，缬草/卡斯卡特混合物提取物通过调节GABA能/5-羟色胺能信号改善与睡眠相关的行为，包括睡眠时间。
