Department of Life Science, College of Natural Sciences and Engineering, Kyonggi University, Suwon, 16227, Republic of Korea.
Thai Nguyen University of Agriculture and Forestry, Quyet Thang, Thai Nguyen, Vietnam.
J Microbiol. 2018 May;56(5):317-323. doi: 10.1007/s12275-018-7454-3. Epub 2018 May 2.
A novel, aerobic, Gram-stain-negative, non-motile, non-spore forming, rod-shaped bacterium, designated strain Dol 15-39, was isolated from a seawater sample near Geoje Island in the South Sea, Republic of Korea. The strain was found to be oxidase-negative and catalase-positive. The isolate was observed to grow at temperatures from 4 to 37°C, at salinities of up to 7%, and at pH levels from 6 to 9; moreover, it was not able to degrade starch, DNA, esculin, or tyrosine. Phylogenetic analysis based on 16S rRNA gene sequences showed that Dol 15-39 was most closely related to Flavobacterium jumunjinense HME7102 with a sequence similarity of 97.3%. However, the levels of DNA-DNA relatedness between Dol 15-39 and the most closely related species were much lower than 70%, confirming that they represented distinct genomic species. The genomic DNA G + C content of Dol 15-39 was calculated to be 32.6 mol%. MK-6 was the predominant respiratory quinine, while iso-C (25.0%), iso-C G (17.0%), and iso-C 3-OH (10.4%) were the major cellular fatty acids. Phosphatidylethanolamine was identified as a major polar lipid, while various unidentified aminolipids and polar lipids were also detected. Based on polyphasic taxonomic data, Dol 15-39 represents a novel species of the genus Flavobacterium, for which the name F. aquimarinum sp. nov. is proposed. The type strain is accessible under the culture collection numbers (KEMB 9005-617 = JCM 31930).
一株新的、好氧的、革兰氏阴性、非运动的、不产芽孢的杆状细菌，被命名为 Dol 15-39 菌株，从韩国南海巨济岛附近的海水样本中分离得到。该菌株氧化酶阴性，过氧化氢酶阳性。该菌在 4 至 37°C 的温度、高达 7%的盐度和 pH 值 6 至 9 的条件下生长；此外，它不能降解淀粉、DNA、七叶灵和酪氨酸。基于 16S rRNA 基因序列的系统发育分析表明，Dol 15-39 与 Flavobacterium jumunjinense HME7102 最为密切相关，序列相似性为 97.3%。然而，Dol 15-39 与最密切相关的物种之间的 DNA-DNA 同源性水平远低于 70%，这证实了它们代表了不同的基因组物种。Dol 15-39 的基因组 DNA G+C 含量计算为 32.6 mol%。MK-6 是主要的呼吸醌，而 iso-C（25.0%）、iso-C G（17.0%）和 iso-C 3-OH（10.4%）是主要的细胞脂肪酸。磷脂酰乙醇胺被鉴定为主要的极性脂，同时还检测到各种未鉴定的胺脂和极性脂。基于多相分类学数据，Dol 15-39 代表了 Flavobacterium 属的一个新种，建议将其命名为 F. aquimarinum sp. nov.。该模式菌株的培养物可在以下编号中获得（KEMB 9005-617 = JCM 31930）。
