Sosa Enrique, Kim Rachel, Rojas Ernesto J, Hosohama Linzi, Hennebold Jon D, Orwig Kyle E, Clark Amander T
Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97006, USA.
Stem Cell Res. 2017 May;21:5-8. doi: 10.1016/j.scr.2017.03.011. Epub 2017 Mar 15.
The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).
恒河猴诱导多能干细胞（riPSC）系UCLAi090 - A（riPSC90）由名为REF90的恒河猴胚胎成纤维细胞（REF）生成。REF90细胞和riPSC90系通过短串联重复序列分析进行了鉴定，具有正常的雄性（42, XY）核型。riPSC90系表达自我更新标志物，包括OCT4、NANOG、TRA - 1 - 81和SSEA4，并在移植到免疫缺陷小鼠后形成畸胎瘤。riPSC90可与riPSC89平行使用，riPSC89源自另一只恒河猴胚胎培养的REFs（索萨等人，2016年）。
