Wu Kun-Chang, Kao Chun-Pin, Ho Yu-Ling, Chang Yuan-Shiun
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
Department of Nursing, Hsin Sheng Junior College of Medical Care and Management, Taoyuan 32544, Taiwan.
Molecules. 2017 Jul 5;22(7):1115. doi: 10.3390/molecules22071115.
is the dried root and rhizome of (L.) Hook. and has been used for centuries in the treatment of inflammation, fever, pneumonia, burns, and various disorders. However, the chromatographic methods to determine the phytochemical composition of have never been reported. This study not only aims to develop a valid high-performance liquid chromatography (HPLC) method and to establish a chromatographic fingerprint for the quality control of , it also establish the proposed content limits of Quercetin, Ugonin J, and Ugonin M. An HPLC method with a RP column (250 × 4.6 mm, 5 μm) was developed for the quantitative analysis of Quercetin, Ugonin J, and Ugonin M in . A simple gradient of (A) methanol/(B) phosphoric acid in water (5-45 min, 70-80% A; 50-55 min, 80-70% A) was used and 360 nm was selected as the detection wavelength. The average contents and proposed content limits for were calculated with a -test and a measurement uncertainty test based on 20 batches of authentic samples. Limits of detection (LOD), quantification (LOQ), linearity, precision, repeatability, stability, and recovery of the developed method were validated. All of the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of Quercetin, Ugonin J, and Ugonin M and to acquire the fingerprints of all of the collected samples. At the 99% confidence level, the calculated content limits were 56.45, 112.15, and 277.98 mg/kg for Quercetin, Ugonin J, and Ugonin M, respectively. Those validated HPLC quantitative method, fingerprinting profile, and the proposed content limits of three chemical markers that could be used in the quality control of in the market.
是（L.）Hook.的干燥根及根茎，数个世纪以来一直用于治疗炎症、发热、肺炎、烧伤及各种病症。然而，尚未见有测定其植物化学成分的色谱方法报道。本研究不仅旨在开发一种有效的高效液相色谱（HPLC）方法并建立色谱指纹图谱用于其质量控制，还确定了槲皮素、乌冈菌素J和乌冈菌素M的规定含量限度。建立了一种采用反相柱（250×4.6 mm，5μm）的HPLC方法用于定量分析中的槲皮素、乌冈菌素J和乌冈菌素M。采用甲醇/水（A）/磷酸（B）（5 - 45分钟，70 - 80% A；50 - 55分钟，80 - 70% A）的简单梯度洗脱，选择360 nm作为检测波长。基于20批正品样品计算其平均含量及规定含量限度，并进行t检验和测量不确定度检验。对所建立方法的检测限（LOD）、定量限（LOQ）、线性、精密度、重复性、稳定性和回收率进行了验证。定量测定和指纹图谱方法的所有验证结果均令人满意。然后将所建立的方法应用于测定槲皮素、乌冈菌素J和乌冈菌素M的含量，并获取所有收集样品的指纹图谱。在99%置信水平下，计算得到的槲皮素、乌冈菌素J和乌冈菌素M的含量限度分别为56.45、112.15和277.98 mg/kg。所验证的HPLC定量方法、指纹图谱及三种化学标志物的规定含量限度可用于市场上的质量控制。
