粪便样本中A组轮状病毒NSP4基因分析及其与基因分型的关联
Analysis of NSP4 Gene and Its Association with Genotyping of Rotavirus Group A in Stool Samples.
作者信息
Teimoori Ali, Nejati Mehrab, Ebrahimi Saeedeh, Makvandi Manoochehr, Zandi Milad, Azaran Azarakhsh
机构信息
Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Iran Biomed J. 2018 Jan 1;22(1):42-9. doi: 10.22034/ibj.22.1.42. Epub 2017 Jul 11.
BACKGROUND
Non-structural protein 4 (NSP4) is a critical protein for rotavirus (RV) replication and assembly. This protein has multiple domains and motifs that predispose its function and activity. NSP4 has a sequence divergence in human and animal RVs. Recently, 14 genotypes (E1-E14) of NSP4 have been identified, and E1 and E2 have been shown to be the most common genotypes in human.
METHODS
The gene and protein sequence of NSP4 in RV-positive samples were inspected with the aim of NSP4 genotyping and variation analysis in viroporin and other domains. P and G typings of RV samples were carried out by WHO primers using a semi-multiplex PCR method. Non-typeable RV samples were amplified by conserved primers and sequenced.
RESULTS
In viroporin and enterotoxin, conserved sequence was detected, and amino acids substitution with the same biochemical properties was found.
CONCLUSION
Association of NSP4 genotype with P or G genotyping G1/G9 correlates with E1 genogroups. In electrophoretyping of RV, E2 genotype had a short pattern when compared to E1.
背景
非结构蛋白4（NSP4）是轮状病毒（RV）复制和组装的关键蛋白。该蛋白具有多个结构域和基序，决定其功能和活性。NSP4在人和动物RV中存在序列差异。最近，已鉴定出NSP4的14种基因型（E1-E14），其中E1和E2是人类中最常见的基因型。
方法
检测RV阳性样本中NSP4的基因和蛋白质序列，目的是对NSP4进行基因分型以及对病毒孔蛋白和其他结构域进行变异分析。使用半多重PCR方法通过世界卫生组织（WHO）引物对RV样本进行P和G分型。通过保守引物对无法分型的RV样本进行扩增并测序。
结果
在病毒孔蛋白和肠毒素中检测到保守序列，并发现具有相同生化特性的氨基酸替代。
结论
NSP4基因型与P或G基因型G1/G9的关联与E1基因群相关。在RV的电泳分型中，与E1相比，E2基因型具有较短的条带模式。
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