Kotowski Ulana, Heiduschka Gregor, Kadletz Lorenz, Fahim Tammer, Seemann Rudolf, Schmid Rainer, Schneider Sven, Mitterbauer Andreas, Thurnher Dietmar
Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, A-1090 Vienna, Austria.
Department of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, A-1090 Vienna, Austria.
Oncol Lett. 2017 Jul;14(1):1147-1151. doi: 10.3892/ol.2017.6189. Epub 2017 May 17.
Thymoquinone (TQ) is the main bioactive constituent present in black seed oil (Nigella sativa); it has shown anti-inflammatory and anti-neoplastic effects in various cancer cell types. The aim of the present study was to investigate the effects of TQ on head and neck squamous cell carcinoma (HNSCC) cell lines, on its own and in combination with radiation and cisplatin, respectively. The SCC25 and CAL27 HNSCC cell lines were treated with TQ alone and in combination with cisplatin or radiation, respectively. Proliferation assays and clonogenic assays were performed. Apoptosis was detected by flow cytometry. TQ exhibited dose-dependent cytotoxicity via apoptosis in the investigated cell lines. In combination with cisplatin, TQ resulted in no significant increase in cytotoxicity. Combined with radiation, TQ significantly reduced clonogenic survival compared with each treatment method alone. TQ is a promising agent in the treatment of head and neck cancer due to its anti-proliferative and radiosensitizing properties. However, the combination of TQ with cisplatin showed no therapeutic benefit .
百里醌（TQ）是黑种草籽油（黑种草）中的主要生物活性成分；它在多种癌细胞类型中显示出抗炎和抗肿瘤作用。本研究的目的是分别研究TQ对头颈鳞状细胞癌（HNSCC）细胞系的作用，以及它与放疗和顺铂联合使用时的作用。SCC25和CAL27 HNSCC细胞系分别单独用TQ处理，以及与顺铂或放疗联合处理。进行了增殖试验和克隆形成试验。通过流式细胞术检测细胞凋亡。在所研究的细胞系中，TQ通过凋亡表现出剂量依赖性细胞毒性。与顺铂联合使用时，TQ并未导致细胞毒性显著增加。与放疗联合使用时，与单独的每种治疗方法相比，TQ显著降低了克隆形成存活率。由于其抗增殖和放射增敏特性，TQ是治疗头颈癌的一种有前景的药物。然而，TQ与顺铂联合使用未显示出治疗益处。
