Mao Liang, Liu Chuanling, Lu Kun, Su Yu, Gu Cheng, Huang Qingguo, Petersen Elijah J
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, P. R. China.
Department of Crop and Soil Sciences, University of Georgia, Griffin, Georgia 30223, United States.
Carbon N Y. 2016 Nov;109:566-574. doi: 10.1016/j.carbon.2016.08.037. Epub 2016 Aug 16.
While graphene has substantial commercial promise, numerous aspects regarding its ecological effects such as its potential for bioaccumulation are not well known. C-labeled few layer graphene (FLG) was dispersed in artificial freshwater and uptake of FLG by , an oligochaete, was assessed. After exposure for 36 h to a 1 mg/L FLG suspension, the FLG body burden in the organism was nearly 60 ng/mg (on a dry mass basis). Multiple characterization results confirmed that the proteins secreted by the organisms during the exposure period coated the FLG, thus increasing its stability and decreasing its size in suspension. Uptake behaviors of exposed to FLG and protein-coated FLG at concentrations of approximately 1 mg/kg or to at 100 μg/L were also quantified. Protein-coated FLG demonstrated different bioaccumulation behaviors for both organisms compared to uncoated FLG, with the FLG body burden in increased but that in reduced. The data provide the first evidence that the proteins secreted by after exposure to FLG can coat FLG, thus increasing the aqueous stability of FLG, decreasing its size, and changing its bioaccumulation potential.
虽然石墨烯具有巨大的商业前景，但其生态效应的许多方面，如生物累积潜力，尚不为人所知。将碳-14标记的少层石墨烯（FLG）分散在人工淡水中，并评估寡毛纲动物对FLG的摄取情况。在暴露于1 mg/L的FLG悬浮液36小时后，生物体中FLG的体内负荷接近60 ng/mg（以干重计）。多项表征结果证实，生物体在暴露期间分泌的蛋白质包裹了FLG，从而提高了其稳定性并减小了其在悬浮液中的尺寸。还对暴露于浓度约为1 mg/kg的FLG和蛋白质包裹的FLG或100 μg/L的[具体生物名称未给出]的摄取行为进行了量化。与未包裹的FLG相比，蛋白质包裹的FLG对两种生物体均表现出不同的生物累积行为，[一种生物名称]中FLG的体内负荷增加，而[另一种生物名称]中则降低。这些数据首次证明，暴露于FLG后[生物名称]分泌的蛋白质可以包裹FLG，从而提高FLG在水中的稳定性，减小其尺寸，并改变其生物累积潜力。
