Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Republic of Korea.
Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Oxid Med Cell Longev. 2017;2017:1289041. doi: 10.1155/2017/1289041. Epub 2017 Jun 14.
Atmospheric-pressure plasma (APP) has received attention due to its generation of various kinds of reactive oxygen/nitrogen species (ROS/RNS). The controllability, as well as the complexity, is one of the strong points of APP in various applications. For biological applications of this novel method, the cytotoxicity should be estimated at various levels. Herein, we suggest red blood cell (RBC) as a good cell model that is simpler than nucleated cells but much more complex than other lipid model systems. Air and N gases were compared to verify the main ROS/RNS in cytotoxicity, and microscopic and spectroscopic analyses were performed to estimate the damages induced on RBCs. The results shown here will provide basic information on APP-induced cytotoxicity at cellular and molecular levels.
由于大气压等离子体（APP）能够产生各种活性氧/氮物质（ROS/RNS），因此受到了关注。在各种应用中，其可控性和复杂性是其优势之一。对于这种新型方法的生物应用，应该在各个层面估计细胞毒性。在这里，我们建议使用红细胞（RBC）作为一种良好的细胞模型，它比有核细胞简单，但比其他脂质模型系统复杂得多。比较了空气和氮气，以验证细胞毒性中的主要 ROS/RNS，并进行了微观和光谱分析，以评估对 RBC 造成的损伤。这里展示的结果将为 APP 诱导的细胞毒性在细胞和分子水平上提供基本信息。
