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海胆核RNA中不同的单拷贝序列集。

Distinct single-copy sequence sets in sea urchin nuclear RNAs.

作者信息

Ernst S G, Britten R J, Davidson E H

出版信息

Proc Natl Acad Sci U S A. 1979 May;76(5):2209-12. doi: 10.1073/pnas.76.5.2209.

DOI:10.1073/pnas.76.5.2209
PMID:287061
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC383567/
Abstract

The purpose of this study was to determine whether nuclear RNAs (nRNA) of sea urchin embryos and adult tissues contain identical or partially distinct sets of single-copy sequence transcripts. A DNA tracer was prepared consisting mainly of sequences absent from gastrula nRNA; 3.6% of this tracer reacted with adult intestine nRNA but not with gastrula nRNA. The existence of a differentially transcribed DNA fraction was verified by its partial purification and rehybridization to intestine and gastrula nRNAs. About one-third of the genomic single-copy sequence is represented in both nRNAs, or about 2 x 10(8) nucleotides. The differentially transcribed portion of the single-copy genome identified in this work includes about 3.5 x 10(7) nucleotides.

摘要

本研究的目的是确定海胆胚胎和成年组织的核RNA（nRNA）是否包含相同或部分不同的单拷贝序列转录本集合。制备了一种DNA示踪剂，其主要由原肠胚nRNA中不存在的序列组成；该示踪剂的3.6%与成年肠nRNA反应，但不与原肠胚nRNA反应。通过对其进行部分纯化并与肠和原肠胚nRNA重新杂交，证实了存在差异转录的DNA部分。基因组单拷贝序列的约三分之一存在于两种nRNA中，即约2×10⁸个核苷酸。在本研究中鉴定出的单拷贝基因组的差异转录部分约为3.5×10⁷个核苷酸。

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本文引用的文献

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Analysis of repeating DNA sequences by reassociation.通过重缔合分析重复DNA序列。
Methods Enzymol. 1974;29:363-418. doi: 10.1016/0076-6879(74)29033-5.
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A measurement of the sequence complexity of polysomal messenger RNA in sea urchin embryos.对海胆胚胎中多聚核糖体信使核糖核酸序列复杂性的测量。
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Globin messenger RNA synthesis and processing during haemoglobin induction in Friend cells. I. Evidence for transcriptional control in clone M2.弗氏细胞血红蛋白诱导过程中的珠蛋白信使核糖核酸合成与加工。I. 克隆M2中转录控制的证据
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Cell. 1975 Jul;5(3):291-9. doi: 10.1016/0092-8674(75)90104-x.
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Structural gene sets active in embryos and adult tissues of the sea urchin.在海胆胚胎和成年组织中活跃的结构基因集。
Cell. 1976 Apr;7(4):487-505. doi: 10.1016/0092-8674(76)90200-2.
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Mouse globin gene expression in erythroid and non-erythroid tissues.小鼠珠蛋白基因在红细胞系和非红细胞系组织中的表达。
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Analysis of the complexity and diversity of mRNA from chicken liver and oviduct.鸡肝脏和输卵管mRNA的复杂性与多样性分析
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