Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, Giza, 12578, Egypt.
Faculty of Environmental Agricultural Sciences, Arish University, Arish, 45511, Egypt.
Sci Rep. 2024 Sep 18;14(1):21763. doi: 10.1038/s41598-024-71758-9.
Metal and metal oxide nanocomposites have unique properties and are promising for antibacterial and anticancer applications. In this work, we aimed to highlight the relationship between the biosynthesis ways of silver and gold-doped zinc oxide nanocomposites and their functions as anticancer on cell lines (MCF-7 and HepG2). The propolis was used to biosynthesize four different nanoparticles with the same components, including zinc, gold and silver. The nanocomposites were characterized using various techniques, including ultraviolet-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Energy Dispersive X-ray analysis (EDX) and cytotoxicity assays. The result of this study showed that formed nanocomposites have a similar level of Zn, Au, and Ag, ranging from 23-34%, 2-6%, and 2-3%, respectively. In addition, adding the components simultaneously produces the fastest color change, and the fabricated nanoparticles have spherical shapes with different layers. In addition, the prepared nanoparticles influenced the cell viability of the cancer cell lines, with the most effective one when Zn, Au, and Ag were added spontaneously to form a nanocomposite called (All) with IC of 24.5 µg/mL for MCF7 cells and 29.1 µg/mL for HepG2 cells. Thus, the study illustrates that the preparation of nanocomposite generated through green synthesis with different methods significantly affects the structure and function and may improve the synthesis of nanocomposite to be developed into an efficacious therapeutic agent for cancers. In addition, this study opens the door toward a novel track in the field of nanocomposites as it links the synthesis with structure and function. Further anti-cancer properties, as well as animal testing are needed for those nanocomposites.
金属和金属氧化物纳米复合材料具有独特的性质，有望在抗菌和抗癌应用中得到应用。在这项工作中，我们旨在强调银和金掺杂氧化锌纳米复合材料的生物合成方式与其在细胞系（MCF-7 和 HepG2）上作为抗癌的功能之间的关系。我们使用蜂胶来生物合成具有相同成分的四种不同纳米粒子，包括锌、金和银。使用各种技术对纳米复合材料进行了表征，包括紫外-可见光谱（UV-Vis）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能量色散 X 射线分析（EDX）和细胞毒性测定。研究结果表明，形成的纳米复合材料具有相似水平的 Zn、Au 和 Ag，分别为 23-34%、2-6%和 2-3%。此外，同时添加这些成分会产生最快的颜色变化，并且所制造的纳米粒子具有不同层的球形形状。此外，制备的纳米粒子影响癌细胞系的细胞活力，其中当 Zn、Au 和 Ag 自发形成称为（All）的纳米复合材料时，效果最为显著，对 MCF7 细胞的 IC 为 24.5µg/mL，对 HepG2 细胞的 IC 为 29.1µg/mL。因此，该研究表明，通过绿色合成用不同方法制备的纳米复合材料的结构和功能会显著影响结构和功能，并可能改善纳米复合材料的合成，以开发出有效的癌症治疗剂。此外，这项研究为纳米复合材料领域开辟了一条新途径，因为它将合成与结构和功能联系起来。这些纳米复合材料还需要进一步的抗癌特性和动物试验。
